Molecular Diversity of Early-Born Subplate Neurons
Molecular Diversity of Early-Born Subplate Neurons
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DOI：
10.1093/cercor/bhs137
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发表时间：
2013-06-01
期刊：
影响因子：
3.7
通讯作者：
Molnar, Zoltan
中科院分区：
文献类型：
--
作者：
Hoerder-Suabedissen, Anna;Molnar, Zoltan
小鼠中的基板细胞通常定义为位于白色物质和层6a之间的基板层中的细胞。它们是大脑皮层中最早出生和成熟的细胞。小鼠出生后的亚板层含有表达突触前蛋白复合物3（Cplx 3）、结缔组织生长因子（CTGF）、孤儿核受体Nr 4a 2（Nurr 1）和G蛋白偶联溶血磷脂酸受体1（Lpar 1/Edg 2）的神经元。所有4种分子标记物均显示在年轻的出生后年龄层6 b限制性表达，CTGF表达在年轻的出生后亚板中最广泛。然而，所有4个标记物在其表达模式中不同程度地重叠。在这里，我们证明了溴脱氧尿苷出生日期，这些分子亚板标记物的任何一个标记的细胞确实是在E11.5或E12.5在小鼠中产生。此外，我们证明了基因表达和细胞出生日期之间的相关性。Lpar 1-GFP细胞优先在E11.5产生，而Cplx 3或Nurr 1阳性细胞在亚板神经发生的2天高峰期（E11.5E12.5）同样产生。我们的研究还表明，与其他亚板神经元相比，早期出生的亚板神经元标记的Cplx 3，Nurr 1和Lpar 1-GFP在出生后第一周后优先存活。
Subplate cells in the mouse are generally defined as cells located in the subplate layer between the white matter and layer 6a. They are some of the earliest born and maturing cells of the cerebral cortex. The postnatal subplate layer in mouse contains neurons with expression of the presynaptic protein complexin 3 (Cplx3), connective tissue growth factor (CTGF), the orphan nuclear receptor Nr4a2 (Nurr1), and the G-protein-coupled lysophosphatidic acid receptor 1 (Lpar1/Edg2). All 4 of these molecular markers show layer 6b-restricted expression at young postnatal ages, with CTGF expression being the most widespread in the young postnatal subplate. However, all 4 markers overlap in their expression pattern to varying degrees. Here we demonstrate with bromodeoxyuridine birthdating that cells labeled with any 1 of these molecular subplate markers are indeed generated at E11.5 or E12.5 in the mouse. Furthermore, we demonstrate a correlation between gene expression and cell birthdates. Lpar1-GFP cells are preferentially generated on E11.5, whereas Cplx3 or Nurr1-positive cells are equally generated during the 2-day peak of subplate neurogenesis (E11.5E12.5). Our study also demonstrates that early-born subplate neurons labeled by Cplx3, Nurr1, and Lpar1-GFP survive preferentially after the first postnatal week compared with other subplate neurons.