RESPIRATORY SYNCYTIAL VIRUS POLYPEPTIDES .5. THE KINETICS OF GLYCOPROTEIN-SYNTHESIS
RESPIRATORY SYNCYTIAL VIRUS POLYPEPTIDES .5. THE KINETICS OF GLYCOPROTEIN-SYNTHESIS
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DOI：
10.1099/0022-1317-66-6-1241
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
LEVINE, S
中科院分区：
文献类型：
--
作者：
GRUBER, C;LEVINE, S
呼吸道合胞病毒的细胞结合糖蛋白包括GP 1（90 K [90，000]）、VP 70（70 K）、VGP 48（48 K）和GP 26（26 K）。病毒蛋白在HeLa细胞中合成的时间过程显示，核衣壳蛋白（VPN 41）是第一个出现在感染后11 h，随后出现的其他病毒蛋白在感染后16 h。用[~ 3 H]亮氨酸或[~（35）S]甲硫氨酸进行的脉冲追踪实验表明，VGP 48和GP 26的前体是70 K的未切割蛋白（VP 70），其需要至少30 min才能追踪到其最终产物，而GP 1的前体是46 K的糖蛋白，也需要至少30 min才能追踪到GP 1。胰蛋白酶处理莫能菌素处理的感染细胞表明，VP 70可以在细胞内裂解成其产物VGP 48和GP 26。
The cell-associated glycoproteins of respiratory synctial virus included GP1 (90K [90,000]), VP70 (70K), VGP48 (48K) and GP26 (26K). The time course of virus protein synthesis in HeLa cells revealed that the nucleocapsid protein (VPN41) was the 1st to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. Pulse-chase experiments with [3H]leucine or [35S]methionine demonstrated that the precursor for VGP48 and GP26 was an uncleaved protein of 70K (VP70) which required at least 30 min to chase into its final products, while the precursor for GP1 was a glycoprotein of 46K, and also required at least 30 min to chase into GP1. Trypsin treatment of monensin-treated infected cells suggested that VP70 can be cleaved intracellularly into its products VGP48 and GP26.