DIRECT MEASUREMENT OF THE EQUILIBRIUM BETWEEN GLUTATHIONE AND DITHIOTHREITOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
DIRECT MEASUREMENT OF THE EQUILIBRIUM BETWEEN GLUTATHIONE AND DITHIOTHREITOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0014-5793(91)81305-r
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发表时间：
1991-10-21
期刊：
影响因子：
3.5
通讯作者：
NELSON, JW
中科院分区：
文献类型：
--
作者：
CHAU, MH;NELSON, JW
用高效液相色谱法直接测定了还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、还原型二硫苏糖醇（DTT（SH）SH）和氧化型二硫苏糖醇（DTT（S）1 S）之间的平衡常数。反应GSSG + DTT（SH）SH half-arrow-right-over-half-arrow-left 2GSH + DTT（S）1 S在25 ℃时的平衡常数从约200 M（低于pH 8）变化到约2800 M（高于pH 11）。所观察到的pH值的依赖性通常是一致的，这些硫醇的酸解离常数的公布值。
The equilibrium constant between reduced glutathione (GSH), oxidized glutathione (GSSG), reduced dithiothreitol (DTT(SH)SH), and oxidized dithiothreitol (DTT(S)1S) has been directly measured by high performance liquid chromatography analysis of equilibrium mixtures. The equilibrium constant at 25-degrees-C for the reaction GSSG + DTT(SH)SH half-arrow-right-over-half-arrow-left 2GSH + DTT(S)1S varies from approximately 200 M, below pH 8, to approximately 2800 M, above pH 11. The observed pH dependence is generally consistent with published values of acid dissociation constants of these thiols.