Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.
Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.
复制标题
DOI：
10.1002/anie.201606060
复制
发表时间：
2016-12-05
影响因子：
16.6
通讯作者：
Cooper, Jonathan M
中科院分区：
文献类型：
--
作者：
Xu, Gaolian;Nolder, Debbie;Reboud, Julien;Oguike, Mary C;van Schalkwyk, Donelly A;Sutherland, Colin J;Cooper, Jonathan M
我们首次展示了使用折纸的折纸技术从全血中多重确定微生物种类，从而实现了DNA提取、环介导的等温扩增(LAMP)和基于阵列的荧光检测的连续步骤。一种低成本的手持手电筒向肉眼显示最终dna扩增子的存在，在45 分钟内从手指刺穿的人体血液中提供“样本到答案”的诊断，而用户干预最少。为了证明该方法，我们展示了三种疟原虫的鉴定，在一项操作员盲法研究中，以金标准聚合酶链式反应分析为基准，分析了80例 患者样本。我们还表明，当使用存储的或固定的参考样本时，该测试保持其诊断准确性。
We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper‐folding technique of origami to enable the sequential steps of DNA extraction, loop‐mediated isothermal amplification (LAMP), and array‐based fluorescence detection. A low‐cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a “sample‐to‐answer” diagnosis from a finger‐prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold‐standard polymerase chain reaction (PCR) assay in an operator‐blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.