Molecular analysis of the RNA and protein components recognized by anti-La(SS-B) autoantibodies.
Molecular analysis of the RNA and protein components recognized by anti-La(SS-B) autoantibodies.
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对抗 La(SS-B) 自身抗体识别的 RNA 和蛋白质成分进行分子分析。
DOI：
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发表时间：
1985
影响因子：
4.6
通讯作者：
I. Mackay
中科院分区：
文献类型：
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作者：
L. Mcneilage;S. Whittingham;I. Jack;I. Mackay
本研究的目的是确定是否与La（SS-B）核抗原的自身抗体的血清与相同或不同的细胞或病毒核糖核蛋白（RNP）的反应，以及是否与抗La（SS-B）的患者包括一个同质组的表型和血清学标记。34个抗La（SS-B）血清研究中检测的过程中，筛选2,000例血清提到疑似或确定的多系统自身免疫性疾病。对从抗La（SS-B）血清的IgG组分与根据其病毒和细胞（非病毒）RNA含量选择的细胞系之间体外形成的免疫复合物中分离的小核（sn）RNP的分子组分进行分析，结果表明，所有34种抗La（SS-B）血清均与同一组细胞RNA和EB病毒编码的两种病毒RNA反应。La（SS-B）RNP含有一个主要的50，000道尔顿抗原多肽，其在二维免疫印迹上分解成5-6个不均匀带电同种。除了抗La（SS-B）的反应性，所有34个血清显示含有抗Ro（SS-A）的活性，通过对流免疫电泳（CIEP），然而，有三个例外，抗原Ro（SS-A）多肽是无法检测到的免疫印迹。该组与抗La（SS-B）的同质性表明，34例中31例（88%）有高丙种球蛋白血症，33例（97%）有类风湿因子，27例（30个测试，90%）是HLA-B8。因此，所有抗La（SS-B）血清均与同一组与抗原性50，000道尔顿核蛋白相关的RNA反应，抗La（SS-B）自身抗体的存在鉴定了一组具有原发性干燥综合征血清学和表型特征的同质患者。
The aim of this study was to determine whether sera with autoantibodies to the La(SS-B) nuclear antigen react with the same or different sets of cellular or viral ribonucleoproteins (RNPs) and whether patients with anti-La(SS-B) comprised a homogeneous group with respect to phenotypic and serological markers. The 34 anti-La(SS-B) sera studied were detected in the course of screening 2,000 sera referred from patients with suspected or defined multisystem autoimmune disease. Analysis of the molecular components of the small nuclear (sn) RNPs isolated from immune complexes developed in vitro between the IgG fractions of the anti-La(SS-B) sera and cell lines selected for their content of viral and cellular (non-viral) RNA showed that all 34 anti-La(SS-B) sera reacted with the same group of cellular RNAs and with two viral RNAs encoded by Epstein-Barr virus. The La(SS-B) RNPs contained one major 50,000 dalton antigenic polypeptide that resolved into 5-6 heterogeneously charged isospecies on two-dimensional immunoblots. In addition to anti-La(SS-B) reactivity, all 34 sera were shown to contain anti-Ro(SS-A) activity by counterimmunoelectrophoresis (CIEP); however, with three exceptions, the antigenic Ro(SS-A) polypeptide was not detectable by immunoblotting. The homogeneity of this group with anti-La(SS-B) was indicated by the findings that of the 34 cases 31 (88%) had hypergammaglobulinaemia, 33 (97%) had rheumatoid factor and 27 (of 30 tested, 90%) were HLA-B8. Thus all anti-La(SS-B) sera react with the same set of RNAs associated with an antigenic 50,000 dalton nucleoprotein, and the presence of anti-La(SS-B) autoantibodies identified a homogeneous group of patients with the serological and phenotypic features of primary Sjögren's syndrome.