Establishment of a mammary stromal fibroblastic cell line for in vitro studies in mice of mammary adipocyte differentiation.
Establishment of a mammary stromal fibroblastic cell line for in vitro studies in mice of mammary adipocyte differentiation.
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建立乳腺基质成纤维细胞系，用于小鼠乳腺脂肪细胞分化的体外研究。
DOI：
10.1095/biolreprod.109.077958
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发表时间：
2010
影响因子：
3.6
通讯作者：
Nakatani H
中科院分区：
文献类型：
--
作者：
Nakatani H
乳腺间质脂肪组织重塑对于妊娠、哺乳和退化期乳腺的正常发育非常重要。然而，乳腺间质脂肪组织重塑的确切机制仍不清楚。我们已经建立了一个乳腺间质，成纤维细胞样细胞系（MSF）从原代小鼠乳腺培养引入一个温度敏感的猿猴病毒-40大肿瘤抗原。在与乳腺发育相关的几种激素中，氢化可的松被发现将MSF细胞提交给前脂肪细胞谱系，而胰岛素被发现诱导细胞的细胞外基质依赖性成脂分化，如通过脂质积累和标记基因表达所评估的。有趣的是，催乳素通过其受体和下游STAT 5抑制了MSF细胞（而不是3 T3-L1细胞）的脂肪诱导的成脂分化。此外，MSF细胞与乳腺上皮细胞HC 11共培养和在HC 11条件培养基中培养也抑制了MSF细胞的成脂分化。我们已经证明，至少一些群体的乳腺基质细胞的成脂分化是由来自上皮细胞的催乳激素和体液因子调节的，这表明这些乳腺细胞的反应可能与其他部位的脂肪细胞不同。我们相信，MSF细胞系将证明是一个有用的模型，阐明乳腺间质脂肪的发展，在体外，以及代表了重要的第一步，发展稳定的脂肪细胞系，忠实地代表其原产地。
Mammary stromal adipose tissue remodeling is important for appropriate mammary gland development during pregnancy, lactation, and involution. However, the precise mechanisms underlying mammary stromal adipose tissue remodeling remain unclear. We have established a mammary stromal, fibroblastlike cell line (MSF) from primary mouse mammary culture by introducing a temperature-sensitive simian virus-40 large tumor antigen. Among several hormones related to mammary gland development, hydrocortisone was found to commit MSF cells to a preadipocyte lineage, whereas insulin was found to induce extracellular matrix-dependent adipogenic differentiation of the cells, as assessed by lipid accumulation and marker gene expression. Interestingly, such hormone-induced adipogenic differentiation of MSF cells, but not 3T3-L1 cells, was suppressed by prolactin through its receptor and downstream STAT5. Furthermore, coculture of MSF cells with mammary epithelial HC11 cells and culture in HC11-conditioned medium also suppressed adipogenic differentiation of MSF cells. We have demonstrated that adipogenic differentiation of at least some populations of mammary stromal cells is modulated by lactogenic hormones and humoral factors from epithelial cells, suggesting that the response of these mammary cells may differ from adipocytes at other sites. We believe that the MSF cell line will prove a useful model to elucidate mammary stromal adipose development in vitro as well as represent an important first step toward developing stable adipocyte cell lines that faithfully represent their site of origin.