Awa1p on the cell surface of sake yeast inhibits biofilm formation and the co-aggregation between sake yeasts and Lactobacillus plantarum ML11-11
Awa1p on the cell surface of sake yeast inhibits biofilm formation and the co-aggregation between sake yeasts and Lactobacillus plantarum ML11-11
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DOI：
10.1016/j.jbiosc.2014.10.007
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发表时间：
2015-05-01
影响因子：
2.8
通讯作者：
Morinaga, Yasushi
中科院分区：
文献类型：
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作者：
Hirayama, Satoru;Shimizu, Masashi;Morinaga, Yasushi
我们检查了植物乳杆菌 ML11-11 与酿酒酵母的发泡和非发泡突变株之间的混合物种生物膜形成。与非泡沫突变体相比，野生型菌株显示出明显较低水平的生物膜形成。 Awa1p 是一种参与清酒酿造过程中泡沫形成的蛋白质，是一种糖基磷脂酰肌醇 (GPI) 锚定蛋白，与清酒酵母的细胞壁相关。无泡沫突变菌株 Kyokai no. 的 AWA1 基因。 701 (K701) 丢失了包含 GPI 锚信号的 C 端序列。野生型菌株 Kyokai no. 的混合物种生物膜形成和共聚集7 (K7) 显着低于 K701 UT-1 (K701) ura3/ura3 trp1/trp1)，而质粒上携带 AWA1 的菌株 K701 UT-1 的水平与 K7 相当。含有缺失 GPI 锚定信号的 AWA1 的菌株 1(701 UT-1) 的生物膜形成和共聚集水平与 K701 UT-1 相似。这些结果清楚地表明，清酒酵母菌株 K7 表面存在的 Awa1p 抑制酵母细胞和植物乳杆菌 ML11-11 之间的粘附，从而阻碍混合物种生物膜的形成。(C) 2014，日本生物技术学会。保留所有权利。
We examined mixed-species biofilm formation between Lactobacillus plantarum ML11-11 and both foaming and non-foaming mutant strains of Saccharomyces cerevisiae sake yeasts. Wild-type strains showed significantly lower levels of biofilm formation compared with the non-foaming mutants. Awa1p, a protein involved in foam formation during sake brewing, is a glycosylphosphatidylinositol (GPI)-anchored protein and is associated with the cell wall of sake yeasts. The AWA1 gene of the non-foaming mutant strain Kyokai no. 701 (K701) has lost the C-terminal sequence that includes the GPI anchor signal. Mixed-species biofilm formation and co-aggregation of wild-type strain Kyokai no. 7 (K7) were significantly lower than K701 UT-1 (K701) ura3/ura3 trp1/trp1), while the levels of strain K701 UT-1 carrying the AWA1 on a plasmid were comparable to those of K7. The levels of biofilm formation and co-aggregation of the strain 1(701 UT-1 harboring AWA1 with a deleted GPI anchor signal were similar to those of K701 UT-1. These results clearly demonstrate that Awa1p present on the surface of sake yeast strain K7 inhibits adhesion between yeast cells and L plantarum ML11-11, consequently impeding mixed-species biofilm formation. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.