ev 3, a structural gene locus for endogenous virus, segregates with the gs+chf+ phenotype in matings of line 63 chickens.
ev 3, a structural gene locus for endogenous virus, segregates with the gs+chf+ phenotype in matings of line 63 chickens.
复制标题
ev 3 是内源病毒的结构基因座，在 63 系鸡的交配中与 gs chf 表型分离。
DOI：
10.1016/0042-6822(79)90031-x
复制
发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
E. Buss
中科院分区：
文献类型：
--
作者：
S. Astrin;L. Crittenden;E. Buss
ev3 is one of seven recently described genetic loci of the chicken which contain structural genes for endogenous virus (S. M. Astrin, 1978,Proc. Nat. Acad. Sci. USA75, 5941–5945).ev3 is present exclusively in chickens of the gs+chf+phenotype, that is, chickens whose cells produce endogenous viral group-specific antigen (gs) and viral envelope protein (chf). The segregation ofev3 was followed in a genetic cross in which the gs+chf+phenotype was also segregating. The progeny of the cross were analyzed for endogenous viral loci by cleavage of embryo DNA with restriction endonucleaseSst1, electrophoretic separation of the resulting fragments, and detection of bands containing viral sequences by hybridization of the DNA to radiolabeled viral RNA. Four endogenous viral loci,ev1,ev3,ev4, andev5, were identified in the progeny of the cross.ev1 was present in all of the progeny.ev4 andev5 were present in progeny of both the gs+chf+and gs−chf−phenotypes and were observed to be genetically linked.ev3 was present exclusively in progeny of the gs+chf+phenotype and all progeny of this phenotype containedev3. From these data, we suggest thatev3 codes for gs and chf in the cells of gs+chf+chickens.
