Novel electrical detection of label-free disease marker proteins using piezoresistive self-sensing micro-cantilevers
Novel electrical detection of label-free disease marker proteins using piezoresistive self-sensing micro-cantilevers
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DOI：
10.1016/j.bios.2004.09.023
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发表时间：
2005-04-15
影响因子：
12.6
通讯作者：
Kim, TS
中科院分区：
文献类型：
--
作者：
Wee, KW;Kang, GY;Kim, TS
我们报道了一种机电生物传感器，用于使用自感压阻微杠杆电检测具有疾病标志物的蛋白质。电检测，通过表面应力的变化，抗原抗体（Ag-Ab）的特异性结合是通过直接的纳米机械响应的微制造的自感微杠杆完成的。压阻式传感器测量相对于由生物分子特异性结合引起的表面应力的膜电阻变化。当特异性结合发生在功能化的。一个表面，表面应力引起整个悬臂，导致悬臂弯曲和压阻层的电阻变化。悬臂梁生物传感器用于检测前列腺特异性抗原（PSA）和C反应蛋白（CRP），这是前列腺癌和心脏疾病的特异性标志物。从上述实验中可以看出，传感器输出电压与注射的抗原浓度（无抗原、10 ng/ml、100 ng/ml、1 μ g/ml）成正比。发现PSA和CRP抗体分别对其抗原具有非常特异性。这表明，自感知微悬臂梁的方法是有益的检测疾病标志物，和我们的压阻微悬臂梁传感器系统是适用于小型化的生物传感器系统。(c)2004 Elsevier B. V.保留所有权利。
We report an electro-mechanical biosensor for electrical detection of proteins with disease markers using self-sensing piezoresistive microcantilevers. Electrical detection, via surface stress changes, of antigen-antibody (Ag-Ab) specific binding was accomplished through a direct nano-mechanical response of micro-fabricated self-sensing micro-cantilevers. A piezoresistive sensor measures the film resistance variation with respect to surface stress caused by biomolecules specific binding. When specific binding occurred on a functionalized. An surface, surface stress was induced throughout the cantilever, resulting in cantilever bending and resistance change of the piezoresistive layer. The cantilever biosensors were used for the detection of prostate specific antigen (PSA) and C-reactive proteins (CRP), which are a specific marker of prostate cancer and cardiac disease. From the above experiment, it was revealed that the sensor output voltage was proportional to the injected antigen concentration (without antigen, 10 ng/ml, 100 ng/ml, 1 mu g/ml). PSA and CRP antibodies were found to be very specific for their antigens, respectively. This indicated that the self-sensing micro-cantilever approach is beneficial for detecting disease markers, and our piezoresistive micro-cantilever sensor system is applicable to miniaturized biosensor systems. (c) 2004 Elsevier B.V. All rights reserved.