Ethylene induced formation of maize cortex Aerenchyma is involved to Programmed Cell Death(PCD)

Ethylene induced formation of maize cortex Aerenchyma is involved to Programmed Cell Death(PCD)
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发表时间:
2000
期刊:
Journal of Central China Normal University
影响因子:
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通讯作者:
Gaohong Wang;Hui Wang;Jiu-Li Huang
Gaohong Wang;Hui Wang;Jiu-Li Huang
中科院分区:
其他
文献类型:
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作者:
Gaohong Wang;Hui Wang;Jiu-Li Huang

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乙烯可以诱导玉米根系皮层细胞的脱落,进而诱导气孔组织的形成。随着乙烯浓度的增加,死亡细胞数量增加。通过TUNEL (terminal deoxyynculleotidyl transferasmedated dUTP nick end labeling)实验发现,皮质细胞的nDNA片段化,DNA的3'OH末端数量增加。这些结果首次提供了乙烯诱导的肺泡形成与PCD有关的直接证据。同时,发现活性氧(ROS)根部SOD和CAT活性明显降低。由于ROS可以诱导PCD,外源性乙烯诱导的PCD可能是由ROS介导的。
Ethylene can induce the deach of cortex cells of Maize root and then induce the formation of Aerenchyma. With the increment of the concentration of ethylene, the number of death cells increases. Using TUNEL (terminal deoxynculeotidyl transferasemediated dUTP nick end labeling) experiment, it is found that nDNA of cortex cell is fragmented and the number of 3′OH terminals of DNA is increased. These results firstly provide directl evidence that ethylene induced formation of Aerechyma involves PCD. At the same time, it was f ound that the activity of SOD and CAT in the root of reactive oxygen species (ROS) decrease clearly . Since ROS can induce PCD, the PCD induced by the extraneous ethelene maybe mediated by ROS.