Effects of pollination and postpollination processes on selfing rate in Mimulus ringens.
Effects of pollination and postpollination processes on selfing rate in Mimulus ringens.
复制标题
授粉和授粉后过程对酸杨自交率的影响。
DOI：
10.3732/ajb.1600145
复制
发表时间：
2016
影响因子：
3
通讯作者：
J. D. Karron
中科院分区：
文献类型：
--
作者：
Yuliya B. Sorin;R. Mitchell;Dorset W. Trapnell;J. D. Karron
研究前提
自交亲和开花植物的群体内和群体间的自交率差异很大。这种变化往往是由于自花和异交花粉沉积在柱头上的数量和时间的差异，以及控制受精成功的授粉后机制的影响。本研究探讨了传粉和授粉后过程在决定猴花，Mimulus ringens自交率的相对重要性。
方法
我们用三种试验处理之一的花粉对17个无关母本的花进行人工授粉，这些试验处理旨在复制田间条件：（1）同时沉积50%的自交花粉和50%的来自5个无关供体的异交花粉;（2）自交花粉，15分钟后施加等量的来自5个无关供体的异交花粉;（3）5个无关供体的异交花粉，15 min后施用等量的自交花粉。我们在8个多态性微卫星位点对757个后代进行了基因分型，并使用亲子排除法来确定每个幼苗是自交还是异交。
关键成果
当自交和异交花粉同时到达时，以及当自交花粉在异交花粉之前15分钟到达时，观察到的自交和异交后代的比例没有偏离预期的1：1比例。然而，当异交花粉施加前15分钟，自交花粉，有一个显着过量的异交后代。
结论
在Mimulus ringens自交率的影响，在花粉到达的时间小的差异，但不是由非随机授粉后排序。
PREMISE OF THE STUDY
Selfing rates vary widely within and among populations of self-compatible flowering plants. This variation is often attributed to differences in the amount and timing of self and outcross pollen deposition on stigmas, as well as to the influence of postpollination mechanisms that control fertilization success. This study explores the relative importance of pollination and postpollination processes in determining selfing rates in monkeyflower, Mimulus ringens.
METHODS
We hand-pollinated flowers on 17 unrelated mothers with pollen from one of three experimental treatments intended to replicate field conditions: (1) simultaneous deposition of 50% self pollen and 50% outcross pollen from 5 unrelated donors; (2) self pollen followed 15 min later by application of an equal amount of outcross pollen from five unrelated donors; and (3) outcross pollen from 5 unrelated donors followed 15 min later by application of an equal amount of self pollen. We genotyped 757 progeny at 8 polymorphic microsatellite loci and used paternity exclusion to determine whether each seedling was selfed or outcrossed.
KEY RESULTS
When self and outcross pollen arrived simultaneously, and when self pollen arrived 15 min prior to outcross pollen, the observed proportions of self and outcross progeny did not deviate from the expected 1:1 ratio. However, when outcross pollen was applied 15 min prior to self pollen, there was a significant excess of outcross progeny.
CONCLUSIONS
Selfing rate in Mimulus ringens is influenced by small differences in the timing of pollen arrival, but not by nonrandom postpollination sorting.