Analysis of the 3' flanking region of the human c-myc gene in lymphomas with the t(8;22) and t(2;8) chromosomal translocations.
Analysis of the 3' flanking region of the human c-myc gene in lymphomas with the t(8;22) and t(2;8) chromosomal translocations.
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分析具有 t(8;22) 和 t(2;8) 染色体易位的淋巴瘤中人类 c-myc 基因的 3 侧翼区域。
DOI：
10.1093/nar/14.10.4037
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发表时间：
1986
影响因子：
14.9
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Sun,LK;Showe,LC;Croce,CM
我们克隆并绘制了c-myc基因38 kb 3'的序列。该区域被发现在本质上是高度重复的，并与BLUR 8alu探针广泛杂交。来自该区域的独特序列探针被用于绘制t（2;8）或t（8;22）易位淋巴瘤细胞系的染色体断点。在5个细胞系（PA682、LY67、LY47、LY66和LY91）中，免疫球蛋白轻链位点易位到c-myc下游大于47kb的区域。在其中一个细胞系JI中，8q+染色体上的断点位于c-myc的25-32 kb 3'。BL2细胞系的断点先前被定位在c-myc癌基因的10 kb 3'处。在染色体断裂点在10kb到47kb以上的细胞系中，c-myc mRNA的稳态水平为淋巴母细胞对照的0.5到10倍。c-myc转录本的不同水平并不是c-myc基因与易位免疫球蛋白轻链位点之间距离的直接作用。
We have cloned and mapped the sequences extending 38 kb 3' of the c-myc gene. This region is found to be highly repetitive in nature and hybridizes extensively with a BLUR 8 Alu probe. Unique sequence probes derived from this region were used to map the chromosomal breakpoints of a number of lymphoma cell lines with t(2;8) or t(8;22) translocations. In five of the cell lines (PA682, LY67, LY47, LY66 and LY91), the immunoglobulin light chain locus translocates into a region which is greater than 47 kb downstream of c-myc. For one of the cell lines, JI, the location of the breakpoint on the 8q+ chromosome was found to be 25–32 kb 3' of c-myc. The breakpoint for the BL2 cell line had been previously mapped at 10 kb 3' of the c-myc oncogene. Analyses of steady-state levels of c-myc mRNA in cell lines with chromosomal break points ranging from 10 kb to greater than 47 kb 3' of c-myc range from 0.5 to 10X the levels in lymphoblast controls. The different levels of c-myc transcripts is not a direct function of the distance between the c-myc gene and the translocated immunoglobulin light chain locus.