Horseradish peroxidase immobilized on aluminum-pillaredinterlayered clay for the catalytic oxidadon of phenolic wastewater
Horseradish peroxidase immobilized on aluminum-pillaredinterlayered clay for the catalytic oxidadon of phenolic wastewater
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DOI：
10.1016/j.watres.2005.11.017
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发表时间：
2006-01-01
期刊：
影响因子：
12.8
通讯作者：
Zuo, P
中科院分区：
文献类型：
--
作者：
Cheng, J;Yu, SM;Zuo, P
以铝柱撑粘土（Al-PILC）为载体固定辣根过氧化物酶（HRP），制备酶-粘土复合物，用于处理含酚废水。固定化的HRP发挥了完美的苯酚去除沉淀或转化为其他产品在较宽的pH范围从4.5至9.3。聚乙二醇（PEG）的加入可以显著提高苯酚的去除效率，并减少所需的固定化酶的量，以达到90%以上的高去除率。当PEG/苯酚的质量比为0.4，过氧化氢/苯酚的摩尔比为1.5时，在无缓冲液存在下，苯酚的氧化反应可在反应开始后的较短保留时间内完成。与游离酶相比，AI-PILC固定化HRP具有更好的贮存稳定性。但是，固定化酶的重复使用性并不令人满意。在第四次重复试验中，固定化酶失去其催化性能。进一步的研究应该集中在提高可重用性上。(c)2005爱思唯尔有限公司保留所有权利。
Horseradish peroxidase (HRP) was successfully immobilized on aluminum-pillared interlayered clay (Al-PILC) to obtain enzyme-clay complex for the treatment of wastewater polluted with phenolic compounds. The immobilized HRP exerted a perfect phenol removal by precipitation or transforming to other products over a broader pH range from 4.5 to 9.3. The addition of polyethylene glycol (PEG) could significantly enhance the phenol removal efficiency, and reduce the amount of immobilized enzyme required to achieve a high removal efficiency of over 90%. When the mass ratio of PEG/phenol and the molar ratio of hydrogen peroxide/phenol were 0.4 and 1.5, respectively, the oxidation of phenol could be completed within short retention time after the initiation of reaction in the absence of buffer. HRP immobilized on AI-PILC had better storage stability compared with free enzyme. However, the reusability of the immobilized enzyme was not very satisfactory. In the fourth repeated test, the immobilized enzyme lost its catalytic performance. Further research should focus on the improvement of reusability. (c) 2005 Elsevier Ltd. All rights reserved.