Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project
Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project
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DOI：
10.1016/s1383-5742(03)00013-9
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发表时间：
2003-03-01
影响因子：
5.3
通讯作者：
Fenech, M
中科院分区：
文献类型：
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作者：
Bonassi, S;Neri, M;Fenech, M
吸烟对人体淋巴细胞微核（MN）频率的影响一直是许多人群研究的目标。在大多数报告中，结果是出乎意料的负面，在许多情况下，吸烟者的MN频率低于非吸烟者。从HUMN国际合作项目的24个数据库的汇总重新分析已进行，目的是了解吸烟习惯对MN频率的影响。完整的数据库包括5710名受试者，其中3501名非吸烟者，1409名当前吸烟者和800名前吸烟者，这些受试者都在职业和环境调查中。重新分析的总体结果证实了当前吸烟者的MN频率略有下降（频率比（FR）= 0.97，95%置信区间（CI）= 0.97）。0.93-1.01）和既往吸烟者（FR = 0.96，95% CI = 0.91-1.01）。在职业性暴露于遗传毒性物质的受试者中，MN频率不受每天吸烟数量的影响，而在非暴露吸烟者中观察到典型的U形曲线，表明每天吸烟30支或更多的个体MN频率显著增加（FR = 1.59，95%CI = 1.35-1.88）。该分析证实，吸烟者不会经历MN频率的总体增加，尽管当考虑到与职业暴露的相互作用时，重度吸烟者是唯一一组显示遗传毒性损伤显著增加（通过淋巴细胞微核试验测量）的人群。从这些结果中，一些一般性的建议，涉及吸烟者的生物监测研究的设计可以制定。应经常收集有关吸烟习惯的定量数据，因为如果没有这些数据，简单地将吸烟者与非吸烟者进行比较可能会产生误导。重度吸烟者亚组（：每天30支香烟）在其数量足以满足统计学要求时，应进行专门评价。应始终检测吸烟习惯和职业性遗传毒性剂暴露之间是否存在相互作用。(C)2003 Elsevier Science B. V.保留所有权利。
The effect of tobacco smoking on the frequency of micronuclei (MN) in human lymphocytes has been the object of many population studies. In most reports, the results were unexpectedly negative, and in many instances smokers had lower frequencies of MN than non-smokers. A pooled re-analysis of 24 databases from the HUMN international collaborative project has been performed with the aim of understanding the impact of smoking habits on MN frequency. The complete database included 5710 subjects, with 3501 non-smokers, 1409 current smokers, and 800 former smokers, among subjects in occupational and environmental surveys. The overall result of the re-analysis confirmed the small decrease of MN frequencies in current smokers (frequency ratio (FR) = 0.97, 95% confidence interval (CI) =. 0.93-1.01) and in former smokers (FR = 0.96, 95% CI = 0.91-1.01), when compared to non-smokers. MN frequency was not influenced by the number of cigarettes smoked per day among subjects occupationally exposed to genotoxic agents, whereas a typical U-shaped curve is observed for non-exposed smokers, showing a significant increase of MN frequency in individuals smoking 30 cigarettes or more per day (FR = 1.59, 95% CI = 1.35-1.88). This analysis confirmed that smokers do not experience an overall increase in MN frequency, although when the interaction with occupational exposure is taken into account, heavy smokers were the only group showing a significant increase in genotoxic damage as measured by the micronucleus assay in lymphocytes. From these results some general recommendations for the design of biomonitoring studies involving smokers can be formulated. Quantitative data about smoking habit should always be collected because, in the absence of such data, the simple comparison of smokers versus non-smokers could be misleading. The sub-group of heavy smokers ( :30 cigarettes per day) should be specifically evaluated whenever it is large enough to satisfy statistical requirements. The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested. (C) 2003 Elsevier Science B.V. All rights reserved.