In vitro sorption and desorption of basic fibroblast growth factor from biodegradable hydrogels
In vitro sorption and desorption of basic fibroblast growth factor from biodegradable hydrogels
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DOI：
10.1016/s0142-9612(98)00089-1
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发表时间：
1998-10-01
期刊：
影响因子：
14
通讯作者：
Ikada, Y
中科院分区：
文献类型：
--
作者：
Tabata, Y;Nagano, A;Ikada, Y
研究了碱性成纤维细胞生长因子（bFGF）与生物可降解明胶水凝胶的体外相互作用，重点研究了bFGF在明胶水凝胶中的吸附和脱附。碱性bFGF被吸附到酸性明胶的水凝胶上，在4 ℃下随着时间的推移等电点（IEP）为5.0，而碱性明胶的IEP为9.0和I型胶原蛋白。bFGF的吸附几乎是独立的吸附温度，除了4摄氏度和水凝胶的水含量。荧光显微镜观察显示，碱性成纤维细胞生长因子被吸附到酸性明胶水凝胶的内部。碱性成纤维细胞生长因子的酸性明胶的结合摩尔比为1.0左右。碱性成纤维细胞生长因子吸附到酸性明胶水凝胶增加时，明胶进一步羧化。bFCF吸附到酸性明胶水凝胶中比吸附到聚（丙烯酸）（PAAc）水凝胶中更慢，可能是因为明胶的负电荷密度较低。碱性成纤维细胞生长因子的吸附随溶液离子强度的增加而降低，表明静电相互作用是碱性成纤维细胞生长因子吸附到酸性明胶水凝胶的主要驱动力。然而，即使在更高的离子强度的溶液中，吸附的碱性成纤维细胞生长因子没有解吸从酸性明胶水凝胶，在对比的PAAc水凝胶。(C)1998由Elsevier Science Ltd.出版。保留所有权利。
In vitro interaction of basic fibroblast growth factor (bFGF) with biodegradable gelatin hydrogels was investigated, focusing on its sorption into the hydrogels and desorption from them. Basic bFGF was sorbed to the hydrogel of acidic gelatin with an isoelectric point (IEP) of 5.0 over time at 4 degrees C, in contrast to that of basic gelatin with an IEP of 9.0 and type I collagen. The bFGF sorption was almost independent of the sorption temperature except for 4 degrees C and the hydrogel water content. Fluorescent microscopic observation revealed that bFGF was sorbed into the interior of the acidic gelatin hydrogel. The binding molar ratio of bFGF to the acidic gelatin was around 1.0. The bFGF sorption to the acidic gelatin hydrogel increased when gelatin was further carboxylated. bFCF was sorbed into the acidic gelatin hydrogel more slowly than into the poly(acrylic acid) (PAAc) hydrogel, probably because of the lower density of negative charge of gelatin. The bFGF sorption decreased with an increase in solution ionic strength, indicating that an electrostatic interaction was the main driving force for bFGF sorption to the acidic gelatin hydrogel. However, even at higher ionic strengths of solution, the sorbed bFGF was not desorbed from the acidic gelatin hydrogel, in contrast to the PAAc hydrogel. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.