Negative electrospray ionization tandem mass spectrometric investigation of ent-kaurane diterpenoids from the genus Isodon.
Negative electrospray ionization tandem mass spectrometric investigation of ent-kaurane diterpenoids from the genus Isodon.
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DOI：
10.1002/jms.1273
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发表时间：
2007-08
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通讯作者：
Yan Zhou;Sheng-Xiong Huang;Li-mei Li-Li-mei-Li-152309092;Jing Yang;Xin Liu;S. Peng;L. Ding;Han-Dong Sun
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文献类型：
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作者：
Yan Zhou;Sheng-Xiong Huang;Li-mei Li-Li-mei-Li-152309092;Jing Yang;Xin Liu;S. Peng;L. Ding;Han-Dong Sun
正戊烷二萜类化合物是从异索东属植物中分离出来的一类天然化合物，具有重要的生物活性。采用负电喷雾串联质谱((-)ESI-MS(n))，通过离子阱仪和esi - q飞行时间（TOF）质谱仪的精确质量测量，研究了c -20非氧合对kauranes和两种c -20氧合对kauranes的破碎模式。分析结果表明，c -20氧合对kauranes的7,20 -epoxy和7,20:14,20 - dipoxy亚群的主要过程是CH(2)O或CO(2)的损失。此外，具有保守核心结构但不同取代基（如羟基、醛、羧基和乙酰基）的c -20-无氧对kauranes化合物，分别通过H(2)O、CO、CO(2)和AcOH的损失产生诊断产物离子。这项工作清楚地证明了串联质谱法在两种主要类型的对戊烷二萜的一系列化合物的诊断碎片化的合理化研究中的实用性。
ent-Kaurane diterpenoids are a class of natural compounds isolated from genus Isodon, which have been found to have important bioactivities. Negative electrospray ionization tandem mass spectrometry ((-)ESI-MS(n)) was used to investigate the fragmentation pattern of C-20-nonoxygenated ent-kauranes and two subtypes of C-20-oxygenated ent-kauranes by using an ion trap instrument and accurate mass measurement on an ESI-Q-time-of-flight (TOF) mass spectrometer. The analysis revealed that loss of CH(2)O or CO(2) is the predominant process for 7, 20-epoxy and 7, 20 : 14, 20-diepoxy subgroup of C-20-oxygenated ent-kauranes. In addition, compounds of C-20-nonoxygenated ent-kauranes with a conserved core structure but different substituent groups, such as a hydroxyl, aldehyde, carboxyl, and acetyl moiety, resulted in diagnostic product ions through losses of H(2)O, CO, CO(2), and AcOH, respectively. This work clearly demonstrates the utility of tandem mass spectrometry for studies on the rationalization of the diagnostic fragmentation of a series of compounds from two main types of the ent-kaurane diterpenoids.