High affinity ligands from in vitro selection:: Complex targets
High affinity ligands from in vitro selection:: Complex targets
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DOI：
10.1073/pnas.95.6.2902
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Gold, L
中科院分区：
文献类型：
--
作者：
Morris, KN;Jensen, KB;Gold, L
使用人红细胞膜作为模型系统以确定通过指数富集（SELEX）方法的配体系统进化（一种用于分离特异性结合几乎任何单一蛋白质的高亲和力寡核苷酸的体外方案）是否可以与潜在靶的复杂混合物一起使用。在选择过程中同时产生针对多个靶的配体，并且这些配体对其靶的结合亲和力与在针对纯靶的类似实验中发现的那些相当。二级选择方案，解卷积-SELEX，促进了配体与混合物中特别感兴趣的靶的快速分离，SELEX为混合物中的多个靶点提供了高亲和力的化合物，并可能成为解剖复杂生物系统的手段。
Human red blood cell membranes were used as a model system to determine if the systematic evolution of ligands by exponential enrichment (SELEX) methodology, an in vitro protocol for isolating high-affinity oligonucleotides that bind specifically to virtually any single protein, could be used with a complex mixture of potential targets, Ligands to multiple targets were generated simultaneously during the selection process, and the binding affinities of these ligands for their targets are comparable to those found in similar experiments against pure targets, A secondary selection scheme, deconvolution-SELEX, facilitates rapid isolation of the ligands to targets of special interest within the mixture, SELEX provides high-affinity compounds for multiple targets in a mixture and might allow a means for dissecting complex biological systems.