ROUTINE DIAGNOSIS OF 7 RESPIRATORY VIRUSES AND MYCOPLASMA-PNEUMONIAE BY ENZYME-IMMUNOASSAY
ROUTINE DIAGNOSIS OF 7 RESPIRATORY VIRUSES AND MYCOPLASMA-PNEUMONIAE BY ENZYME-IMMUNOASSAY
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DOI：
10.1016/0166-0934(94)90166-x
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发表时间：
1994-12-01
影响因子：
3.1
通讯作者：
BURRELL, CJ
中科院分区：
文献类型：
--
作者：
KOK, T;MICKAN, LD;BURRELL, CJ
应用8孔微条复合酶免疫分析法快速检测7种呼吸道病毒和M。pnueumberly。这些病毒包括甲型和B型流感病毒、副流感病毒1型、2型和3型、腺病毒和呼吸道合胞病毒。在61个月期间-1988年6月至1993年6月- 17326个呼吸道标本，从三个州提交，由ELA进行了测试。样本主要来自儿科人群（医院和私人医生）。检出的病毒以RSV为主，其次为腺病毒、副流感病毒3型、M.肺炎、甲型流感、副流感2型、流感B型和副流感1型。封闭抗体的使用证实了病原体的鉴定，特别是对于吸光度值大于一种以上感染性病原体的截止值的样品。不同的方法处理标本，以获得一个均匀的悬浮液，并解释非特异性反应，进行了讨论。与病毒培养相比，该测定显示出85%的平均灵敏度和99%的特异性。该系统为忙碌的快速诊断提供了一种有效的方法。
A composite EIA, using 8-well microstrips, was used for the rapid detection of seven respiratory viruses and M. pnueumoniae. The viruses included influenza A and B, parainfluenza 1, 2 and 3, adenovirus and respiratory syncytial virus. During the 61 month period - June 1988 to June 1993 - 17326 respiratory specimens, submitted from three states, were tested by this ELA. The specimens were mainly from a paediatric population (hospitals and private physicians). RSV was the predominant virus detected, followed by adenovirus, parainfluenza 3, M. pneumoniae, influenza A, parainfluenza 2, influenza B and parainfluenza 1. The use of blocking antibodies confirmed the identification of the agents, in particular with samples showing absorbance values greater than the cutoff with more than one infectious agent. Different methods for processing specimens in order to obtain a uniform suspension, and interpretation of non-specific reactions, are discussed. The assays showed an average sensitivity of 85% and specificity of 99%, compared to virus culture. This ELA system provided an efficient method for the rapid diagnosis of viral and mycoplasmal infections in a busy diagnostic laboratory.