Specific antiviral effect of violaceoid E on bovine leukemia virus
Specific antiviral effect of violaceoid E on bovine leukemia virus
复制标题
DOI：
10.1016/j.virol.2021.06.010
复制
发表时间：
2021-07-06
期刊：
影响因子：
3.7
通讯作者：
Tsukamoto, Kenji
中科院分区：
文献类型：
--
作者：
Murakami, Hironobu;Murakami-Kawai, Makoto;Tsukamoto, Kenji
牛白血病病毒（BLV）感染已经在世界范围内蔓延，在畜牧业中造成重大的经济损失。在BLV高流行率的国家，最大限度地减少经济损失是具有挑战性的;因此，研究各种对策对于改善BLV控制非常重要。由于抗BLV药物尚未开发，本研究探索了具有抗BLV活性的有希望的化合物。最初，化合物库的筛选揭示了从真菌中分离的类紫素E（vioE）显示出抗病毒活性。进一步分析表明，vioE的抗病毒作用抑制了BLV的转录激活。细胞热位移试验和下拉试验提供了vioE和病毒反式激活蛋白Tax之间直接相互作用的证据。这些数据表明，干扰Tax依赖性转录可能是开发抗BLV药物的新靶点。因此，vioE是一种新的抗BLV病毒化合物。
Bovine leukemia virus (BLV) infection has spread worldwide causing significant economic losses in the livestock industry. In countries with a high prevalence of BLV, minimizing economic losses is challenging; thus, research into various countermeasures is important for improving BLV control. Because anti-BLV drugs have not been developed, the present study explored a promising chemical compound with anti-BLV activity. Initially, screening of a chemical compound library revealed that violaceoid E (vioE), which is isolated from fungus, showed antiviral activity. Further analysis demonstrated that the antiviral effect of vioE inhibited transcriptional activation of BLV. Cellular thermal shift assay and pulldown assays provided evidence for a direct interaction between vioE and the viral transactivator protein, Tax. These data indicate that interference with Tax-dependent transcription could be a novel target for development of anti-BLV drugs. Therefore, it is suggested that vioE is a novel antiviral compound against BLV.