Macromolecular Crowding Effect on Cartilaginous Matrix Production: A Comparison of Two-Dimensional and Three-Dimensional Models
Macromolecular Crowding Effect on Cartilaginous Matrix Production: A Comparison of Two-Dimensional and Three-Dimensional Models
复制标题
大分子拥挤效应对软骨基质产生的影响：二维和三维模型的比较
DOI：
10.1089/ten.tec.2012.0408
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发表时间：
2013-08-01
影响因子：
3
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Chen, Bo;Wang, Bin;Liu, Wei
大分子拥挤 (MMC) 已被证明对单层细胞培养模型中细胞外基质的产生和成熟具有有益影响。为了探索其在组织工程中的潜力，使用Ficoll 70和Ficoll 400的混合物来检查其MMC对单层培养的猪软骨细胞的软骨基质生产以及使用猪软骨细胞和聚乙醇酸无纺纤维的体外工程化软骨形成的影响。结果表明，MMC在单层培养细胞（二维模型）中增强了总胶原蛋白和糖胺聚糖的产生，但在体外工程软骨（三维模型）中基质产生和组织形成却受到大分子的显着抑制。对该现象的进一步机制研究对于MMC在再生医学中的应用具有重要意义。
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is used to examine its MMC effect on cartilaginous matrix production of monolayer cultured porcine chondrocytes as well as on in vitro engineered cartilage formation using porcine chondrocytes and polyglycolic acid-unwoven fibers. The results showed that the production of total collagens and glycos-aminoglycans production was enhanced by MMC in monolayer cultured cells (two-dimensional model), but the matrix production and tissue formation were significantly inhibited in the in vitro engineered cartilage (three-dimensional model) by the macromolecules. Further mechanism study on this phenomenon will be important for MMC applications in regenerative medicine.