Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma
Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma
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DOI：
10.3324/haematol.10864
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发表时间：
2007-08-01
期刊：
影响因子：
10.1
通讯作者：
Marafioti, Teresa
中科院分区：
文献类型：
--
作者：
Roncador, Giovanna;Garcia Verdes-Montenegro, Jose-Francisco;Marafioti, Teresa
Background and ObjectivesIn the present paper we report that SAP an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers.Design and MethodsExpression of SAP and PD-1 was investigated by immunohistochemistry in石蜡包埋的组织切片和细胞系中。 Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells.ResultsScreening on more than 500 lymphoma biopsies showed that 95% (40/42) of cases of AITL expressed at least one of these markers. SAP 也在许多急性 T 淋巴细胞白血病病例 (15/21) 中表达，与其在皮质胸腺细胞中的存在一致。 However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells.Interpretation and ConclusionsThese two markers provide additional evidence that AITL arises from germinal center T cells.它们也可能在这种疾病的诊断中具有价值，因为在这种疾病中很少观察到阴性反应。
Background and ObjectivesIn the present paper we report that SAP an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers.Design and MethodsExpression of SAP and PD-1 was investigated by immunohistochemistry in paraffin-embedded tissue sections and in cell lines. Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells.ResultsScreening on more than 500 lymphoma biopsies showed that 95% (40/42) of cases of AITL expressed at least one of these markers. SAP was also expressed on many cases (15/21) of acute T lymphoblastic leukemia, in keeping with its presence in cortical thymocytes. However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells.Interpretation and ConclusionsThese two markers provide additional evidence that AITL arises from germinal center T cells. They may also prove of value in the diagnosis of this disease since a negative reaction was rarely observed in this disorder.