Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling
Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling
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DOI：
10.1242/dev.189670
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发表时间：
2021-03-01
期刊：
影响因子：
4.6
通讯作者：
Wang, Yongsheng
中科院分区：
文献类型：
--
作者：
Wang, Mengyun;Du, Yue;Wang, Yongsheng
在哺乳动物中，精子携带的调节剂可以在受精过程中转移到卵母细胞上，对原核的形成、受精卵的第一次卵裂、植入前胚胎的发育乃至个体出生后的新陈代谢具有不同的影响。精子microRNAs(MiRNAs)在牛着床前胚胎发育中的调控作用尚未见详细报道。通过构建和筛选miR-202表达文库，我们发现miR-202在牛精子中高度富含。作为miR-202的靶基因，共注射SEPT7 siRNA可以部分逆转miR-202抑制剂加速的牛胚胎第一次卵裂。此外，miR-202模拟物和SEPT7 siRNA都延迟了体细胞核移植(SCNT)胚胎的第一次卵裂，表明miR-202-SEPT7介导了牛胚胎第一次卵裂的延迟。通过进一步探讨胚胎发育过程中miR-202/SEPT7、HDAC6与乙酰化α-微管蛋白的关系，我们研究了精子携带的miR-202如何通过SEPT7调控牛胚胎的第一次卵裂过程，并展示了精子携带的miRNAs提高SCNT效率的潜力。
In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, the first cleavage of zygotes, the development of preimplantation embryos and even the metabolism of individuals after birth. The regulatory role of sperm microRNAs (miRNAs) in the development of bovine preimplantation embryos has not been reported in detail. By constructing and screening miRNA expression libraries, we found that miR-202 was highly enriched in bovine sperm. As a target gene of miR-202, co-injection of SEPT7 siRNA can partially reverse the accelerated first cleavage of bovine embryos caused by miR-202 inhibitor. In addition, both a miR-202 mimic and SEPT7 siRNA delayed the first cleavage of somatic cell nuclear transfer (SCNT) embryos, suggesting that miR-202-SEPT7 mediates the delay of first cleavage of bovine embryos. By further exploring the relationship between miR-202/SEPT7, HDAC6 and acetylated a-tubulin during embryonic development, we investigated how sperm-borne miR-202 regulates the first cleavage process of bovine embryos by SEPT7 and demonstrate the potential of sperm-borne miRNAs to improve the efficiency of SCNT.