Oocytes are required for the preantral granulosa cell to cumulus cell transition in mice
Oocytes are required for the preantral granulosa cell to cumulus cell transition in mice
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DOI：
10.1016/j.ydbio.2007.02.019
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发表时间：
2007-05-01
影响因子：
2.7
通讯作者：
Eppig, J. J.
中科院分区：
文献类型：
--
作者：
Diaz, F. J.;Wigglesworth, K.;Eppig, J. J.
窦前颗粒细胞在窦形成后分化为卵丘细胞。卵丘细胞，而不是PAGC，有能力进行扩展。本文报道的实验测试了卵母细胞和FSH在腔前颗粒细胞向卵丘细胞过渡中各自的作用。PAGC-卵母细胞复合物与或不与低剂量的FSH（0.005IU/ml）一起培养，并且分离的PAGC与或不与卵母细胞一起培养。在培养结束时，测试复合物或分离的PAGC响应EGF或FSH（0.5IU/ml）经历卵丘扩增和上调扩增转录物的能力。在培养过程中，对EGF的反应需要卵母细胞而不是FSH的存在。同样，从性腺功能减退小鼠的卵巢分离的复合物，缺乏循环促性腺激素，经历了扩展响应EGF，但不是FSH。相反，激活MAPK 3/1和MAPK 14并响应FSH进行扩增的能力需要预先暴露于低剂量的FSH。然而，这些低水平（0.005或0.025 IU FSH/ml）抑制了Slc 38 a3和Amh的表达，这两种转录本在卵丘细胞中高度表达，表明FSH对卵丘细胞分化的相反作用。总之，在发育过程中，接受FSH扩增的能力需要事先暴露于FSH，而卵母细胞衍生因子本身就足以促进接受EGF扩增的能力。这些结果强调了卵母细胞在驱动PAGC分化为卵丘细胞在窦前到窦卵泡过渡的关键作用。(c)2007爱思唯尔公司All rights reserved.
Preantral granulosa cells (PAGCs) differentiate into cumulus cells following antrum formation. Cumulus cells, but not PAGCs, are competent to undergo expansion. Experiments reported here tested the respective roles of both oocytes and FSH in the transition of preantral granulosa cells to cumulus cells competent to undergo expansion. PAGC-oocyte complexes were cultured with or without a low dose of FSH (0.005 IU/ml) and isolated PAGCs were cultured with or without oocytes. At the end of culture, complexes or isolated PAGCs were tested for their ability to undergo cumulus expansion and upregulate expansion transcripts in response to EGF or FSH (0.5 IU/ml). The ability to undergo expansion in response to EGF required the presence of oocytes but not FSH during the culture period. Likewise, complexes isolated from the ovaries of hypogonadal mice, which lack circulating gonadotropins, underwent expansion in response to EGF, but not FSH. In contrast, the ability to activate MAPK3/1 and MAPK14 and undergo expansion in response to FSH required prior exposure to low doses of FSH. However, these low levels (0.005 or 0.025 IU FSH/ml) suppressed expression of Slc38a3 and Amh, two transcripts highly expressed in cumulus cells, suggesting opposing effects of FSH on cumulus cell differentiation. In conclusion, the ability to undergo expansion in response to FSH requires prior exposure to FSH during development, while oocyte-derived factors alone are sufficient to promote the ability to undergo expansion in response to EGF. These results highlight the crucial role of oocytes in driving the differentiation of PAGCs into cumulus cells during the preantral to antral follicle transition. (c) 2007 Elsevier Inc. All rights reserved.