Anatomical distribution and biochemical characterization of the novel RFamide peptide 26RFa in the human hypothalamus and spinal cord
Anatomical distribution and biochemical characterization of the novel RFamide peptide 26RFa in the human hypothalamus and spinal cord
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DOI：
10.1111/j.1471-4159.2006.04090.x
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发表时间：
2006-10-01
影响因子：
4.7
通讯作者：
Chartrel, Nicolas
中科院分区：
文献类型：
--
作者：
Bruzzone, Federica;Lectez, Benoit;Chartrel, Nicolas
26 RFa是一种新的RFamide肽，最初在两栖动物脑中分离。26 RFa前体随后在各种哺乳动物物种中被表征，但直到现在，哺乳动物（特别是人类）的CNS中产生的26 RFa的解剖学分布和分子形式是未知的。在本研究中，我们已经调查了26 RFa样免疫反应（LI）在人类中枢神经系统的两个区域-下丘脑和脊髓的本地化和生化特性。免疫组织化学标记，使用特异性抗体对人类26 RFa和原位杂交组织化学显示，在人类下丘脑26 RFa表达神经元位于室旁核和腹内侧核。在脊髓中，在背角和侧角观察到26个RFa表达神经元。26 RFa相关肽的表征表明，在人下丘脑和脊髓中存在两种不同的26 RFa分子形式，即26 RFa和43个氨基酸的N-末端延长形式，称为43 RFa。这些数据提供了第一个证据，证明26 RFa和43 RFa实际上是在人类CNS中产生的。26 RF-LI的分布提示26 RFa和/或43 RFa可能参与摄食、性行为和伤害性刺激传递的调节。
26RFa is a novel RFamide peptide originally isolated in the amphibian brain. The 26RFa precursor has been subsequently characterized in various mammalian species but, until now, the anatomical distribution and the molecular forms of 26RFa produced in the CNS of mammals, in particular in human, are unknown. In the present study, we have investigated the localization and the biochemical characteristics of 26RFa-like immunoreactivity (LI) in two regions of the human CNS - the hypothalamus and the spinal cord. Immunohistochemical labeling using specific antibodies against human 26RFa and in situ hybridization histochemistry revealed that in the human hypothalamus 26RFa-expressing neurons are located in the paraventricular and ventromedial nuclei. In the spinal cord, 26RFa-expressing neurons were observed in the dorsal and lateral horns. Characterization of 26RFa-related peptides showed that two distinct molecular forms of 26RFa are present in the human hypothalamus and spinal cord, i.e. 26RFa and an N-terminally elongated form of 43 amino acids designated 43RFa. These data provide the first evidence that 26RFa and 43RFa are actually produced in the human CNS. The distribution of 26RF-LI suggests that 26RFa and/or 43RFa may modulate feeding, sexual behavior and transmission of nociceptive stimuli.