Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in primary astrocyte cultures
Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in primary astrocyte cultures
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DOI：
10.1016/j.febslet.2004.10.074
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发表时间：
2004-12-03
期刊：
影响因子：
3.5
通讯作者：
Ientile, R
中科院分区：
文献类型：
--
作者：
Campisi, A;Caccamo, D;Ientile, R
本研究的目的是评估星形胶质细胞培养物（14 DIV）中谷氨酸诱发的转氨酶（TGase）上调中氧化应激的参与。24小时暴露于谷氨酸引起剂量依赖性耗尽谷胱甘肽细胞内的内容，并增加了细胞培养物中的活性氧产生。这些作用是受体介导的，如GYKI 52466的抑制作用所示。与谷胱甘肽乙酯或半胱胺的预孵育恢复了氧化状态，并有效地显著降低了谷氨酸增加的组织TGase。这些数据表明，组织TGase上调可能是一个生化反应的一部分，氧化应激诱导的星形胶质细胞培养物长期暴露于谷氨酸。(C)2004年由Elsevier B.V.代表欧洲生物化学学会联合会出版。
The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. These effects were receptor-mediated, as demonstrated by inhibition with GYKI 52466. The pre-incubation with glutathione ethyl ester or cysteamine recovered oxidative status and was effective in significantly reducing glutamate-increased tissue TGase. These data suggest that tissue TGase upregulation may be part of a biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to glutamate. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.