GLIAL GROWTH-FACTOR AND NERVE-DEPENDENT PROLIFERATION IN THE REGENERATION BLASTEMA OF URODELE AMPHIBIANS
GLIAL GROWTH-FACTOR AND NERVE-DEPENDENT PROLIFERATION IN THE REGENERATION BLASTEMA OF URODELE AMPHIBIANS
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DOI：
10.1016/0092-8674(86)90394-6
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发表时间：
1986-04-25
期刊：
影响因子：
64.5
通讯作者：
KINTNER, CR
中科院分区：
文献类型：
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作者：
BROCKES, JP;KINTNER, CR
有尾目两栖动物截肢后，胚体细胞（再生的祖细胞）的分裂取决于神经供应提供的一种或多种未知的生长因子。在这里，我们表明，胶质细胞生长因子（GGF），促有丝分裂蛋白先前纯化的牛脑垂体，是存在于蝾螈神经系统提取物。在前肢再生芽基的提取物中也可以检测到，并且在去神经支配后其水平降低。我们先前已经证明，分裂依赖于神经的胚基细胞被称为22/18的单克隆抗体标记。当去神经胚体培养的存在下，部分纯化的GGF从蝾螈脑，或纯GGF从牛垂体，胸腺嘧啶核苷标记指数的胚体细胞是22/18阳性增加多达7倍。这些数据表明，GGF在芽基中的神经依赖性增殖中起作用。
After amputation of a limb from Urodele amphibians, division of the blastemal cells (the progenitor cells of the regenerate) depends on one or more unidentified growth factors provided by the nerve supply. Here we show that glial growth factor (GGF), a mitogenic protein previously purified from the bovine pituitary, is present in newt nervous system extracts. It is also detectable in extracts of the forelimb regeneration blastema, and its level there decreases after denervation. We have previously shown that blastemal cells dependent on the nerve for division are marked by a monoclonal antibody called 22/18. When denervated blastemas are cultured in the presence of partially purified GGF from newt brain, or pure GGF from the bovine pituitary, the thymidine labeling index of blastemal cells that are 22/18-positive is increased as much as sevenfold. These data indicate that GGF plays a role in nerve-dependent proliferation in the blastema.