Plasmodium RON12 localizes to the rhoptry body in sporozoites
Plasmodium RON12 localizes to the rhoptry body in sporozoites
复制标题
疟原虫 RON12 定位于子孢子的棒状体
DOI：
10.1016/j.parint.2018.10.001
复制
发表时间：
2019
影响因子：
1.9
通讯作者：
Tsuboi Takafumi
中科院分区：
文献类型：
--
作者：
Oda-Yokouchi Yuki;Tachibana Mayumi;Iriko Hideyuki;Torii Motomi;Ishino Tomoko;Tsuboi Takafumi
顶复门寄生虫对宿主细胞的入侵是由位于寄生虫入侵形式顶端的微丝、棒状体和致密颗粒分泌细胞器释放的蛋白质介导的。杆状体分泌的蛋白质与宿主细胞受体建立相互作用，并诱导棒状体细胞器的胞吐作用。棒状体蛋白参与靶细胞侵入以及寄生虫在宿主细胞内发育期间驻留的寄生虫空泡的形成。在疟原虫裂殖子中，棒状体颈蛋白（罗恩）复合物由RON 2、RON 4和RON 5组成，并与顶端膜抗原1（AMA 1）相互作用，作为侵入移动连接的关键结构。已知PfRON 12定位于裂殖子的棒状体颈部，但其功能仍不清楚。罗恩蛋白在子孢子中的作用尚不清楚，子孢子是疟原虫的第二种侵入形式，具有保守的顶端分泌结构。在这里，我们证实RON 12在啮齿动物疟疾寄生虫的裂殖子的棒状体颈部表达，而相比之下，我们表明RON 12定位于子孢子的棒状体。伯氏疟原虫RON 12破坏突变体的表型分析显示RON 12在孢子繁殖、蚊子唾液腺和小鼠肝细胞的侵袭以及肝细胞中的发育中是不稳定的。
Invasion of host cells by apicomplexan parasites is mediated by proteins released from microneme, rhoptry, and dense granule secretory organelles located at the apical end of parasite invasive forms. Microneme secreted proteins establish interactions with host cell receptors and induce exocytosis of the rhoptry organelle. Rhoptry proteins are involved in target cell invasion as well as the formation of the parasitophorous vacuole in which parasites reside during development within the host cell. InPlasmodiummerozoites, the rhoptry neck protein (RON) complex consists of RON2, RON4, and RON5, and interacts with apical membrane antigen 1 (AMA1) as a critical structure of the invasion moving junction. PfRON12 is known to localize to the rhoptry neck of merozoites, but its function remains obscure. The roles of RON proteins are largely unknown in sporozoites, the second invasive form ofPlasmodiumwhich possesses a conserved apical end secretory structure. Here, we confirm that RON12 is expressed in the rhoptry neck of merozoites in rodent malaria parasites, whereas in contrast we show that RON12 is localized to the rhoptry body in sporozoites. Phenotypic analysis ofPlasmodium berghei ron12-disrupted mutants revealed that RON12 is dispensable for sporogony, invasion of mosquito salivary glands and mouse hepatocytes, and development in hepatocytes.