SEGMENTAL DISTRIBUTION AND PEPTIDE CONTENT OF PRIMARY AFFERENT NEURONS INNERVATING THE UROGENITAL ORGANS AND COLON OF MALE-RATS
SEGMENTAL DISTRIBUTION AND PEPTIDE CONTENT OF PRIMARY AFFERENT NEURONS INNERVATING THE UROGENITAL ORGANS AND COLON OF MALE-RATS
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DOI：
10.1002/cne.903190411
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发表时间：
1992-05-22
影响因子：
2.5
通讯作者：
DEGROAT, WC
中科院分区：
文献类型：
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作者：
KEAST, JR;DEGROAT, WC
任何内脏传入神经元都含有肽，肽已被提议作为特定靶点的神经通路的组织化学标记物或作为递质候选物。本研究探讨了前一种可能性。初级传入神经元的项目，膀胱，远端结肠或阴茎的大鼠，和结肠的猫被标记与逆行转运的荧光染料（固蓝，真蓝，或荧光金）。在将染料注射到器官中后1至6周，移除腰骶背根神经节，用秋水仙碱处理，并进行5种肽的免疫组织化学鉴定。染料标记的神经元分布在下腰骶神经节，结肠传入神经元几乎只发现在S1神经节，阴茎神经元主要在L 6，和膀胱神经元在这两个水平的器官特异性的方式。P物质（SP）、降钙素基因相关肽（CGRP）、血管活性肠肽（VIP）、脑啡肽（ENK）和生长抑素（SOM）免疫反应性（IR）在所有腰骶神经节的神经元中检测到，但只有这些肽中的一些存在于大比例的标记神经元中。分布于各器官的含肽神经元数量依次为CGRP > SP > VIP > ENK > SOM，但在腰骶神经节和不同器官之间，这些神经元的相对比例存在一定差异。在上腰椎水平观察到的主要差异是在SP-IR神经元之间，其在膀胱和结肠传入神经元中是常见的（25-30%），但在阴茎神经元中不存在。在下腰骶水平，CGRP-IR在结肠神经元中的比例（70%）大于供应膀胱（52%）或阴茎（35%）的神经元。VIP-IR在每组的较少（2-12%）神经元中被发现。猫结肠的传入神经支配表现出类似的肽分布，但具有较少数量的CGRP-IR神经元（45%对70%）和较大数量的VIP-（18%对2%）和ENK-IR（5%对0-2%）神经元。SOM-IR很少发现在任何染料标记的神经元，因此可能是更重要的感觉供应的其他盆腔器官或躯体器官。总之，这些研究表明，初级传入神经元供应的雄性大鼠的泌尿生殖器官和结肠在其节段性分布不同，也表现出一些差异的细胞含有某些肽（SP，CGRP，VIP）的百分比。
any visceral afferent neurons contain peptides, which have been proposed as histochemical markers for nerve pathways of particular targets or as transmitter candidates. The former possibility was investigated in the present study. Primary afferent neurons which project to the urinary bladder, distal colon or penis of rats, and the colon of cats were labelled with retro-gradely transported fluorescent dyes (Fast Blue, True Blue, or Fluoro Gold). One to six weeks after dye injection into the organs, lumbosacral dorsal root ganglia were removed, treated with colchicine, and processed for immunohistochemical identification of five peptides. Dye-labelled neurons were distributed in an organ-specific manner in the lower lumbosacral ganglia, where colon afferent neurons were almost exclusively found in S1 ganglia, penis neurons primarily in L6, and bladder neurons at both levels. Substance P- (SP), calcitonin gene-related peptide-(CGRP), vasoactive intestinal peptide- (VIP), enkephalin- (ENK), and somatostatin- (SOM) immunoreactivity (IR) were detected in neurons in all lumbosacral ganglia but only some of these peptides were present in a large percentage of labelled neurons. The numbers of peptide-containing neurons innervating each organ were CGRP > SP > VIP > ENK > SOM; however some differences were observed in the relative proportions of these neuronal populations between upper lumbar and lower lumbosacral ganglia and between different organs. The major difference seen at the upper lumbar level was amongst the SP-IR neurons, which were common (25-30%) amongst bladder and colon afferent neurons but absent in penis neurons. At the lower lumbar-sacral level CGRP-IR was present in a greater proportion (70%) of colon neurons than neurons which supplied the bladder (52%) or penis (35%). VIP-IR was found in fewer (2-12%) neurons of each group. The afferent innervation of the cat colon exhibited a similar peptide distribution but with smaller numbers of CGRP-IR neurons (45% vs. 70% and larger numbers of VIP- (18% vs. 2%) and ENK-IR (5% vs. 0-2%) neurons. SOM-IR was rarely found in any dye-labelled neurons and may therefore be more important in the sensory supply of other pelvic organs or of somatic organs. In summary, these studies have revealed that primary afferent neurons supplying the urogenital organs and colon of male rats differ in their segmental distribution and also exhibit some differences in the percentage of cells containing certain peptides (SP, CGRP, and VIP).