Secreted Frizzled-related protein 4 inhibits the regeneration of hair follicles
Secreted Frizzled-related protein 4 inhibits the regeneration of hair follicles
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分泌的卷曲相关蛋白4抑制毛囊再生
DOI：
10.7717/peerj.6153
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发表时间：
2019-01-04
期刊：
影响因子：
2.7
通讯作者：
Li, Yuhong
中科院分区：
文献类型：
--
作者：
Guo, Haiying;Xing, Yizhan;Li, Yuhong
分泌型卷曲相关蛋白4（Secreted Frizzled-related Protein 4，sFRP 4）属于Wnt抑制剂。以前，我们报道sFRP 4抑制黑素细胞的分化。在此，我们通过免疫组化染色，发现sFRP 4在人和小鼠毛囊中均有表达，尤其是在外根鞘和内根鞘中。为了揭示sFRP 4在毛囊生长和毛发周期中的作用，我们通过脱毛诱导小鼠背部皮肤的同步毛发周期，并将sFRP 4注射到皮肤中。通过苏木素和伊红染色，我们发现sFRP 4抑制毛囊的再生。然而，毛囊的结构保持完整。与磷酸盐缓冲液处理的毛囊相比，sFRP 4处理的毛囊仍具有相同的角蛋白表达模式。我们的研究结果表明，sFRP 4抑制但不阻断毛囊的再生，并提供了一个潜在的治疗应用，以治疗毛囊再生障碍。
Secreted Frizzled-related Protein 4 (sFRP4) belongs to Wnt inhibitors. Previously, we reported that sFRP4 inhibited the differentiation of melanocyte. Here, by using of immunostaining, we showed that sFRP4 is expressed in both human and mouse hair follicles, especially in the outer root sheath and inner root sheath. To reveal the role of sFRP4 in hair follicle growth and hair cycle, we induced synchronized hair cycle in the dorsal skin of mice by depilation, and injected sFRP4 intradermally into the skin. By hematoxylin and eosin staining, we found that the regeneration of hair follicles was inhibited by sFRP4. However, the structure of hair follicles remained complete. Compared with phosphate buffer saline-treated hair follicles, the sFRP4-treated hair follicles still had the same expression pattern of keratins. Our findings reveal that sFRP4 inhibits but not blocks the regeneration of hair follicles, and supply a potential therapeutic application to treat hair follicle regeneration disorders.