Super-Resolution Optical Lithography with DNA
Super-Resolution Optical Lithography with DNA
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DOI：
10.1021/acs.nanolett.9b01873
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发表时间：
2019-09-01
期刊：
影响因子：
10.8
通讯作者：
Lee, Tae-Hee
中科院分区：
文献类型：
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作者：
Kim, Shi Ho;Liu, Yu;Lee, Tae-Hee
我们开发了一种有效的，通用的，可访问的超分辨率显微镜方法，以低于光学衍射极限的空间分辨率构建纳米颗粒组装体。该方法利用DNA和光活化的DNA交联剂。超分辨率光学技术仅被用作在光衍射极限以下进行测量的手段。此外，目前没有光学技术可用于以几十纳米（nm）的分辨率构建具有精确设计的形状和内部结构的纳米颗粒组装体。在这里，我们证明，我们可以通过利用DNA发夹结合单分子荧光共振能量转移（smFRET）显微镜和光活化的DNA交联剂的自发结构动力学来满足这一不足。由于DNA发夹的自发折叠和展开运动而产生的随机荧光闪烁使我们能够精确地定位折叠的发夹，并且仅当它处于预先设计的目标区域内时才将其固化，该区域的大小低于衍射极限。由于该方法基于光学显微镜和易于点击的DNA交联试剂，因此它将提供一种有效的手段来以光学超分辨率创建具有形状和内部结构的大型纳米颗粒组装体，为研究其物理和光电特性以及开发新型器件打开了广阔的机会窗口。
We developed an efficient, versatile, and accessible super-resolution microscopy method to construct a nanoparticle assembly at a spatial resolution below the optical diffraction limit. The method utilizes DNA and a photo-activated DNA cross-linker. Super-resolution optical techniques have been used only as a means to make measurements below the light diffraction limit. Furthermore, no optical technique is currently available to construct nanoparticle assemblies with a precisely designed shape and internal structure at a resolution of a few tens of nanometers (nm). Here we demonstrate that we can fulfill this deficiency by utilizing spontaneous structural dynamics of DNA hairpins combined with single-molecule fluorescence resonance energy transfer (smFRET) microscopy and a photoactivated DNA cross-linker. The stochastic fluorescence blinking due to the spontaneous folding and unfolding motions of DNA hairpins enables us to precisely localize a folded hairpin and solidify it only when it is within a predesigned target area whose size is below the diffraction limit. As the method is based on an optical microscope and an easily clickable DNA cross-linking reagent, it will provide an efficient means to create large nanoparticle assemblies with a shape and internal structure at an optical super-resolution, opening a wide window of opportunities toward investigating their photophysical and optoelectronic properties and developing novel devices.