Evolution of cocoa flavanol analytics: impact on reporting and cross-study comparison
Evolution of cocoa flavanol analytics: impact on reporting and cross-study comparison
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DOI：
10.1039/d1fo00215e
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发表时间：
2021-03-26
期刊：
影响因子：
6.1
通讯作者：
Kwik-Uribe, Catherine
中科院分区：
文献类型：
--
作者：
Bussy, Ugo;Ottaviani, Javier, I;Kwik-Uribe, Catherine
可可黄烷醇 (CF) 是一组膳食生物活性物质，二十多年来一直在研究其潜在的健康益处。目前，多种 CF 测试方法已经发展，导致报告的 CF 含量可能存在差异。临床研究中使用的食品和测试材料中 CF 含量的可靠表征对于随时间推移的研究比较至关重要，对于支持生物活性物质饮食建议所需的系统评价和荟萃分析也至关重要。在这项工作中，我们比较了两种广泛应用于表征临床研究中使用的材料的分析方法和 AOAC 新认可的 CF 分析官方方法。当比较用这些方法测定的 CF 含量时，观察到准确度存在 -36% 至 +20% 的差异，这支持了不同方法测定的 CF 值不可直接比较的观点。为了解决差异，开发了线性回归模型来预测 CF 值。该方法经过交叉验证，并直接应用于有关 CF 益处的重要科学论文中发表的 CF 值的转换。这项工作提供了一个有效的工具来比较这些不同方法报告的 CF 值，并能够对调查 CF 生物活性的研究进行比较和解释。
Cocoa flavanols (CF) are a group of dietary bioactives that have been studied for their potential health benefits for over two decades. In this time, multiple methods for CF testing have evolved, introducing the potential for differences in reported CF content. The reliable characterization of CF content in food and test materials used in clinical studies is critical to comparisons of research studies over time, as well as critical to enabling the systematic reviews and meta-analyses required to support dietary recommendations of bioactives. In this work, we compared two analytical methods that have been widely applied to characterize materials used in clinical research and a method newly recognized by AOAC as the official method for CF analysis. Differences in accuracy of -36% to +20% were observed when comparing CF contents determined with these methods, supporting the notion that CF values determined across methods are not directly comparable. To address differences, a linear regression model was developed to predict CF values. This approach was cross-validated and directly applied to the conversion of CF values published in key scientific papers on the benefits of CF. This work provides a valid tool to compare CF values reported across these different methods and enables comparisons and interpretation of studies investigating the bioactivity of CF.