Knockdown Resistance Mutations in Aedes aegypti (Diptera: Culicidae) From Puerto Rico
Knockdown Resistance Mutations in Aedes aegypti (Diptera: Culicidae) From Puerto Rico
复制标题
DOI：
10.1093/jme/tjw115
复制
发表时间：
2016-11-01
影响因子：
2.1
通讯作者：
Flores, Adriana E.
中科院分区：
文献类型：
--
作者：
Ponce-Garcia, Gustavo;Del Rio-Galvan, Samantha;Flores, Adriana E.
埃及伊蚊是拉丁美洲和加勒比地区登革热、寨卡病毒和基孔肯雅病毒的主要传播媒介，对氯氰菊酯的抗药性在埃及伊蚊中广泛存在。对拟除虫菊酯产生抗性的一种常见机制--击倒抗性(Kdr)--是通过昆虫的电压依赖性钠通道的突变而产生的。在这种蚊子中，已经报道了在拟除虫菊酯抗性品系中电压门控钠通道基因(Vgsc)大约10个替换替换。其中两个突变名为Ile1016和Cys1534，广泛存在于拉丁美洲和加勒比海地区的蚊子种群中。这项研究评估了8个Ae对氯氰菊酯的抗性水平和两个kdr突变的频率。2013年在波多黎各采集的埃及伊蚊种群。氯氰菊酯抗性系数是新奥尔良标准菌株的33-214倍。KdR突变Ile1016的频率从0.65到固定(1.0)，Cys1,534的频率从0.8到固定。令人担忧的是，两个群体--卡罗莱纳和卡瓜斯--在两个基因座上都达到了固定。我们的结果表明，氯氰菊酯对Ae.在这些来自波多黎各的收藏品中，埃及伊蚊的控制受到了损害。
Permethrin resistance is widespread in Aedes aegypti (L.), the main dengue, zika, and chikungunya virus vector in Latin America and the Caribbean. A common mechanism of resistance to pyrethroids-knockdown resistance (kdr)-is conferred through mutations in the insect's voltage-dependent sodium channel. In this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. Two of these mutations, named Ile1,016 and Cys1,534, are widespread in mosquito populations from Latin America and the Caribbean. This study assessed the levels of permethrin resistance and the frequency of two kdr mutations in eight Ae. aegypti populations collected in Puerto Rico in 2013. Permethrin resistance factors ranged from 33-214-fold relative to the New Orleans reference strain. The frequency of kdr mutation Ile1,016 ranged from 0.65 to fixation (1.0), and for Cys1,534 frequencies varied from 0.8 to fixation. Alarmingly, two populations-Carolina and Caguas-reached fixation at both loci. Our results suggest that permethrin effectiveness for Ae. aegypti control is compromised in these collections from Puerto Rico.