Establishing optimal selenium status: results of a randomized, double-blind, placebo-controlled trial
Establishing optimal selenium status: results of a randomized, double-blind, placebo-controlled trial
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DOI：
10.3945/ajcn.2009.28169
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发表时间：
2010-04-01
影响因子：
7.1
通讯作者：
Fairweather-Tait, Susan J.
中科院分区：
文献类型：
--
作者：
Hurst, Rachel;Armah, Charlotte N.;Fairweather-Tait, Susan J.
背景资料：膳食建议硒不同的国家之间，主要是因为最佳的硒status.Objective的定义的不确定性：其目的是检查不同形式的selenium.Design的剂量反应关系：一个随机的，双盲的，安慰剂对照的饮食干预进行了119名健康的男性和女性年龄5064 y生活在英国。每天服用安慰剂或含50、100或200 μ g硒（约60%硒代蛋氨酸）的富硒酵母片，富硒洋葱粉（约66% γ-谷氨酰-甲基硒代半胱氨酸，相当于50 μ g Se/d），或未富硒洋葱粉，持续12周。测定血小板谷胱甘肽过氧化物酶活性和血浆硒及硒蛋白P浓度的变化。所有受试者的平均基线血浆硒浓度为95.7 +/- 11.5 ng/mL，10周后显著增加至稳态浓度118.3 +/- 13.1，152.0 +/- 24.3，而每日摄入50、100和200 μ g硒酵母者分别为177.4 ± 26.3 ng/mL。血小板谷胱甘肽过氧化物酶活性没有显着变化，无论是剂量或形式的硒。在所有硒干预组中，硒蛋白P显著增加，从总体基线平均值4.99 +/- 0.80 μ g/mL增加到摄入50，100，或200 μ g硒酵母/d和50 μ g富硒洋葱/d.Conclusions：血浆硒蛋白P是一个有用的生物标志物的状态在人群中相对低硒摄入量，因为它响应于不同的饮食形式的硒。为了优化血浆硒蛋白P浓度，在这项研究中，50微克硒/天，除了习惯摄入量约55微克/天。在血浆硒与癌症风险和死亡率之间的既定关系的背景下，并认识到硒蛋白P的重要功能，这些结果提供了重要的证据，以得出估计的平均需求量硒在成人。本试验在clinicaltrials.gov上注册为NCT 00279812。美国临床营养杂志2010; 91：923-31。
Background: Dietary recommendations for selenium differ between countries, mainly because of uncertainties over the definition of optimal selenium status.Objective: The objective was to examine the dose-response relations for different forms of selenium.Design: A randomized, double-blind, placebo-controlled dietary intervention was carried out in 119 healthy men and women aged 5064 y living in the United Kingdom. Daily placebo or selenium-enriched yeast tablets containing 50, 100, or 200 mu g Se (approximate to 60% selenomethionine), selenium-enriched onion meals (approximate to 66% gamma-glutamyl-methylselenocysteine, providing the equivalent of 50 mu g Se/d), or unenriched onion meals were consumed for 12 wk. Changes in platelet glutathione peroxidase activity and in plasma selenium and selenoprotein P concentrations were measured.Results: The mean baseline plasma selenium concentration for all subjects was 95.7 +/- 11.5 ng/mL, which increased significantly by 10 wk to steady state concentrations of 118.3 +/- 13.1, 152.0 +/- 24.3, and 177.4 +/- 26.3 ng/mL in those who consumed 50, 100, or 200 mu g Se-yeast/d, respectively. Platelet glutathione peroxidase activity did not change significantly in response to either dose or form of selenium. Selenoprotein P increased significantly in all selenium intervention groups from an overall baseline mean of 4.99 +/- 0.80 mu g/mL to 6.17 +/- 0.85, 6.73 +/- 1.01, 6.59 +/- 0.64, and 5.72 +/- 0.75 mu g/mL in those who consumed 50, 100, or 200 mu g Se-yeast/d and 50 mu g Se-enriched onions/d, respectively.Conclusions: Plasma selenoprotein P is a useful biomarker of status in populations with relatively low selenium intakes because it responds to different dietary forms of selenium. To optimize the plasma selenoprotein P concentration in this study, 50 mu g Se/d was required in addition to the habitual intake of approximate to 55 mu g/d. In the context of established relations between plasma selenium and risk of cancer and mortality, and recognizing the important functions of selenoprotein P, these results provide important evidence for deriving estimated average requirements for selenium in adults. This trial was registered at clinicaltrials.gov as NCT00279812. Am J Clin Nutr 2010; 91: 923-31.