Development and polymorphism of simple sequence repeat DNA markers for Bruguiera gymnorrhiza (L.) Lamk
Development and polymorphism of simple sequence repeat DNA markers for Bruguiera gymnorrhiza (L.) Lamk
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木榄简单序列重复DNA标记的开发和多态性
DOI：
10.1046/j.1471-8286.2003.00360.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
S. Changtragoon
中科院分区：
文献类型：
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作者：
T. Sugaya;H. Yoshimaru;T. Takeuchi;M. Katsuta;K. Fujimoto;S. Changtragoon
建立了木榄的微卫星标记。161个克隆中有54个含有微卫星重复序列。根据其中20个克隆的序列数据设计引物对。其中，7条引物对来自日本伊洛摩特岛的32个个体具有多态性。每个位点检测到2 ~ 5个等位基因，杂合度为0.031 ~ 0.500。7种引物中的5种对白茅双歧杆菌和细小菌双歧杆菌的跨种扩增效果也很好。由于我们先前的研究报告了同一Iriomote群体中等位酶的遗传多样性水平非常低，这些微卫星标记应该成为各种遗传分析的有力工具。
Microsatellite markers of Bruguiera gymnorrhiza were developed. Fifty-four of 161 clones were found to contain microsatellite repeats. Primer pairs were designed for 20 of these clones according to their sequence data. Of these, seven primers showed polymorphism for 32 individuals from Iriomote Island, Japan. Two to five alleles per locus were detected, and the observed heterozygosities ranged from 0.031 to 0.500. Cross-species amplification using five of the seven primers also worked well for B. cylindrica and B. parviflora. Because our previous study reported very low levels of genetic diversity for allozymes in the same Iriomote population, these microsatellite markers should be a powerful tool for various kinds of genetic analysis.