Isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (Vigna unguiculata (L.) Walp.) seedling growth
Isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (Vigna unguiculata (L.) Walp.) seedling growth
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DOI：
10.1007/s11274-009-0293-y
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发表时间：
2010-07-01
影响因子：
4.1
通讯作者：
Pandey, Ashok
中科院分区：
文献类型：
--
作者：
Deepa, C. K.;Dastager, Syed G.;Pandey, Ashok
从印度西部高特森林非根际土壤中分离的四种潜在解磷肠杆菌的功效。这些菌株的植物生长促进能力在豇豆进行了评价。所有的都是革兰氏阴性，杆状，0.8-1.6毫米大小，和嗜冷的性质，生长从5至40摄氏度（最佳温度）。28 +/-2 ℃）。所有分离株均在pH 6-12的宽范围内生长，最适pH为7.0，并耐受高达7%（w/v）的盐浓度。16 S rRNA基因测序显示分离株确认为产气肠杆菌属（NII-0907和NII-0929）、阴沟肠杆菌亚种（NII-0907和NII-0929）。Clobacter sp.（NII-0931）和Enterobacter asburiae sp.（NII-0934），它们与之具有>99%的序列相似性。在体外条件下，所有四个菌株被发现产生吲哚乙酸，P-增溶和氰化氢。P-增溶活性与伴随的培养基pH的降低（pH 7.0- 7.0）相一致。
The efficacy of four potential phosphate solubilizing Enterobacter isolated from non-rhizospheric soil in Western ghat forest in India. Plant growth promoting ability of these isolates was evaluated in cowpea. All are gram negative, rod shaped, 0.8-1.6 mm in size, and psychrotrophic in nature, grow from 5 to 40 degrees C (optimum temp. 28 +/- 2 degrees C). All isolates exhibits growth at a wide range of pH 6-12, optimum at pH 7.0 and tolerates up to 7% (w/v) salt concentration. 16S rRNA gene sequencing reveals the confirmation of isolates to Enterobacter aerogenes sp. (NII-0907 and NII-0929), Enterobacter cloacae subsp. cloacae sp. (NII-0931) and Enterobacter asburiae sp. (NII-0934) with which they share >99% sequence similarity. Under in vitro conditions, all the four isolates were found to produce indole acetic acid, P-solubilization and hydrogen cyanide. The P-solubilizing activity coincided with a concomitant decrease in pH of the medium (pH 7.0-