The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000
The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000
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DOI：
10.1073/pnas.1731982100
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发表时间：
2003-09-02
影响因子：
11.1
通讯作者：
Collmer, A
中科院分区：
文献类型：
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作者：
Buell, CR;Joardar, V;Collmer, A
我们报告了模式细菌病原菌假单胞菌致病变种番茄DC 3000（DC 3000）的全基因组序列，该菌对番茄和拟南芥具有致病性。DC 3000基因组（6.5兆碱基）包含一个环状染色体和两个质粒，共同编码5，763个ORF。我们确定了298个已建立和推定的毒力基因，包括几个基因簇编码31个确认和19个预测的III型分泌系统效应蛋白。许多毒力基因是旁系同源家族的成员，并且也接近移动的元件，其共同构成DC 3000基因组的7%。这种细菌拥有大量的转运蛋白，用于获取营养物质，特别是糖，以及与附着在植物表面有关的基因。超过12%的基因致力于调控，这可能反映了在附生生长和发病过程中遇到的不同环境的快速适应的需要。比较分析证实了与两种测序的假单胞菌（恶臭假单胞菌和铜绿假单胞菌）的高度相似性，但发现了DC 3000特有的1,159个基因，其中811个缺乏已知的功能。
We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.