Comparative Genome Sequence Analysis of Actinobacillus pleuropneumoniae Serovar 8 Isolates From Norway, Denmark, and the United Kingdom Indicates Distinct Phylogenetic Lineages and Differences in Distribution of Antimicrobial Resistance Genes.
Comparative Genome Sequence Analysis of Actinobacillus pleuropneumoniae Serovar 8 Isolates From Norway, Denmark, and the United Kingdom Indicates Distinct Phylogenetic Lineages and Differences in Distribution of Antimicrobial Resistance Genes.
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DOI：
10.3389/fmicb.2021.729637
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发表时间：
2021
影响因子：
5.2
通讯作者：
Angen Ø
中科院分区：
文献类型：
--
作者：
Cohen LM;Bossé JT;Stegger M;Li Y;Langford PR;Kielland C;Klem TB;Gulliksen SM;Ranheim B;Grøntvedt CA;Angen Ø
胸膜肺炎放线杆菌是猪胸膜肺炎的病原，这是一种严重影响全球猪健康、福利和生产力的疾病。8型血清型（APP）是挪威和英国（UK）的主要临床血清型，并已从丹麦的临床病例中分离出来。本研究的主要目的是表征挪威人群中胸膜肺炎杆菌APP8分离株的遗传变异性。次要目标是确定APP8在宿主内的变异性；比较挪威、丹麦和英国的APP8细菌种群，包括抗微生物药物耐药性（AMR）基因谱，并评估各国抗微生物药物使用差异和限制动物运动对耐药性发生的影响。采用全基因组测序方法对1983 - 2020年间从英国（n=67）、丹麦（n=22）和挪威（n=123）采集的APP8分离株进行比较。为了研究个体宿主的遗传变异，对从6头挪威猪肺中分离的另外104株APP8进行了比较。在宿主内观察到非常低的变异（≤2个单核苷酸多态性）。来自76个畜群的123株挪威APP8分离株的系统发育显示出一定的种群内遗传变异，但存在明显的地理聚类。当推断三个国际APP8集合的相关性时，拓扑结构突出了两个不同的单系分支的存在，分别以挪威和英国分离物为特征。三个丹麦分离株分散在英国分支中，而其余19个丹麦分离株聚集在两个单系群中，嵌套在挪威分支中。聚结分析，用于估计从一个共同祖先的分歧，表明最后一个共同祖先在几个世纪前。系统发育分析还揭示了AMR基因发生的显著差异，因为这些基因在英国分离株中的流行率是挪威分离株的23倍。增进对人口战略影响的了解有助于监测和控制传染病。
Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a disease of major impact on pig health, welfare, and productivity globally. Serovar 8 (APP) is the predominant clinical serovar in Norway and the United Kingdom (UK), and has been isolated from clinical cases in Denmark. The primary objective of this study was to characterize the genetic variability of isolates of A. pleuropneumoniae APP8 in the Norwegian population. The secondary objectives were to determine the within-host variability of APP8; to compare the APP8 bacterial populations in Norway, Denmark, and the UK, including antimicrobial resistance (AMR) gene profiles and to assess the effect of national differences in antimicrobial drug use and restricted animal movement on the occurrence of resistance. Isolates of APP8 from the UK (n=67), Denmark (n=22), and Norway (n=123) collected between 1983 and 2020 were compared using whole genome sequencing. To investigate genetic variability within individual hosts, an additional 104 APP8 isolates from the lungs of six Norwegian pigs were compared. Very low within-host variation was observed (≤ 2 single nucleotide polymorphisms). The phylogeny of 123 Norwegian APP8 isolates from 76 herds revealed some within-herd genetic variation, but substantial geographical clustering. When inferring the relatedness of the three international APP8 collections, the topology highlighted the existence of two distinct monophyletic branches characterized by the Norwegian and UK isolates, respectively. Three Danish isolates were scattered across the UK branch, whereas the remaining 19 Danish isolates clustered in two monophyletic groups nested in the Norwegian branch. Coalescence analysis, performed to estimate the divergences from a common ancestor, indicated a last common ancestor several centuries ago. The phylogenetic analyses also revealed striking differences in occurrence of AMR genes, as these were 23-times more prevalent among the UK isolates than among the Norwegian isolates. An increased understanding of the effects of population strategies is helpful in surveillance and control of infectious diseases.
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影响因子：
0.8
作者：
Bosse, Janine T.;Li, Yanwen;Li, Yanwen
通讯作者：
Li, Yanwen
DOI：
10.1128/genomea.01667-15
发表时间：
2016-01-28
期刊：
Genome announcements
影响因子：
--
作者：
Bossé JT;Chaudhuri RR;Li Y;Leanse LG;Fernandez Crespo R;Coupland P;Holden MT;Bazzolli DM;Maskell DJ;Tucker AW;Wren BW;Rycroft AN;Langford PR
通讯作者：
Langford PR
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
2.2
作者：
Li, Y.;Bosse, J. T.;Langford, P. R.
通讯作者：
Langford, P. R.