Uniform small graphene oxide as an efficient cellular nanocarrier for immunostimulatory CpG oligonucleotides.
Uniform small graphene oxide as an efficient cellular nanocarrier for immunostimulatory CpG oligonucleotides.
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DOI：
10.1021/am5012595
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发表时间：
2014-04
影响因子：
9.5
通讯作者：
Jinli Sun;J. Chao;Jing Huang;Min Yin;H. Zhang;Cheng Peng;Zengtao Zhong;Nan Chen
中科院分区：
文献类型：
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作者：
Jinli Sun;J. Chao;Jing Huang;Min Yin;H. Zhang;Cheng Peng;Zengtao Zhong;Nan Chen
氧化石墨烯（GO）作为一种有前途的纳米材料，在生物医学研究和应用方面受到越来越多的关注。在这项研究中，我们探索了GO作为合成DNA链的纳米载体的能力。免疫刺激性CpG寡脱氧核苷酸（ODN）连接到聚-L-赖氨酸（PLL）官能化的多分散GO或均匀的小GO（sGO）纳米片。两种类型的GO-CpG ODN纳米缀合物都可以递送到小鼠Raw 264.7巨噬细胞中并具有免疫刺激活性，而sGO-CpG似乎是更有效的刺激剂。此外，与较大的GO-CpG对应物相比，sGO-CpG纳米片表现出更高的细胞摄取但更好的生物相容性。此外，PLL功能化的sGO-CpG比叠氮化物功能化的sGO-CpG具有更高的免疫刺激活性。总之，我们的研究提供了证据表明，sGO可以用作合成单链DNA的理想细胞内纳米载体，并且sGO-PLL-CpG缀合物可以用作潜在的促炎治疗工具。
Graphene oxide (GO) has attracted more and more attention as a promising nanomaterial in biomedical research and applications. In this study, we explore the ability of GO as nanocarrier for synthetic DNA strands. Immunostimulatory CpG oligodeoxynucleotides (ODNs) are attached to Poly-L-Lysine (PLL) functionalized, polydisperse GO, or uniform small GO (sGO) nanosheets. Both types of GO-CpG ODN nanoconjugates can be delivered into murine Raw264.7 macrophages and possess immunostimulatory activity, while sGO-CpG appears to be a more efficient stimulator. In addition, sGO-CpG nanosheets exhibit higher cellular uptake but better biocompatibility compared to the larger GO-CpG counterpart. Furthermore, PLL functionalized sGO-CpG has higher immunostimulatory activity than azide functionalized sGO-CpG. Together, our studies provide evidence that sGO can be utilized as an ideal intracellular nanocarrier for synthetic single-stranded DNA, and sGO-PLL-CpG conjugates may serve as a potential proinflammatory therapeutic tool.