ENHANCEMENT AND INHIBITION OF LUMINOL CHEMILUMINESCENCE BY PHENOLIC-ACIDS
ENHANCEMENT AND INHIBITION OF LUMINOL CHEMILUMINESCENCE BY PHENOLIC-ACIDS
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DOI：
10.1002/bio.1170100306
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发表时间：
1995-05-01
期刊：
影响因子：
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通讯作者：
GARCIA, JAG
中科院分区：
文献类型：
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作者：
DIAZ, AN;SANCHEZ, FG;GARCIA, JAG
我们探讨了一系列酚酸作为鲁米诺化学发光的增强剂或抑制剂的行为，通过三种不同的方法，以确定是否与酚酸结构和氧化还原特性的行为。在加入鲁米诺之前，六氰合铁（III）与酚酸反应，酚酸均抑制化学发光。这些化合物的氧化还原特性明显与结构有关。当酚酸-鲁米诺混合物引发铁氰化物-鲁米诺-O-2化学发光时，某些酚酸类物质表现出化学发光增强作用。和其它作为抑制剂。我们提出了一个机制来解释这些发现。我们发现酚酸的氧化还原特性和鲁米诺-过氧化氢-过氧化物酶体系的化学发光的增强或抑制之间的直接关系，我们提出的机制来解释这些现象。
We explored the behaviour of a series of phenolic acids used as enhancers or inhibitors of luminol chemiluminescence by three different methods to determine if behaviour was associated with phenolic acid structure and redox character. All the phenolic acids inhibited chemiluminescence when hexacyanoferrate(lll) was reacted with the phenolic acids before adding luminol. The redox character of these compounds was clearly related to structure. When hexacyanoferrate(III)-luminol-O-2 chemiluminescence was initiated by phenolic acid-luminol mixtures some phenolic acids behaved as enhancers of chemiluminescence. and others as inhibitors. We propose a mechanism to explain these findings. We found direct relationships between the redox character of the phenolic acids and the enhancement or inhibition of the chemiluminescence of the luminol-H2O2-peroxidase system and we propose mechanism to explain these phenomena.