Focused Ultrasound Modulates the Level of Cortical Neurotransmitters: Potential as a New Functional Brain Mapping Technique
Focused Ultrasound Modulates the Level of Cortical Neurotransmitters: Potential as a New Functional Brain Mapping Technique
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DOI：
10.1002/ima.20284
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Yoo, Seung-Schik
中科院分区：
文献类型：
--
作者：
Min, Byoung-Kyong;Yang, Po Song;Yoo, Seung-Schik
脑皮层神经递质水平的区域调节将作为一种新的功能性脑映射技术来询问大脑的神经化学活动。我们研究了应用低强度、脉冲超声聚焦（FUS）对大脑进行超声处理以调节细胞外多巴胺（DA）和5-羟色胺（5-HT）水平的效用。将FUS递送至大鼠丘脑区，并使用微透析技术从额叶提取细胞外DA和5-HT。采用高效液相色谱法测定DA和5-HT的浓度变化。我们观察到的DA和5-HT的细胞外浓度在FUS处理组与unsonicated组相比，显着增加。我们的研究结果提供了第一个直接的证据，FUS超声处理改变这些单胺神经递质的细胞外浓度的水平，并有潜在的调节作用，对它们的局部释放，摄取或降解。我们的研究结果表明，脉冲应用FUS提供了一个可能的非侵入性调制的神经递质的新的前景，并可能有诊断和治疗的DA/5-HT介导的神经和精神疾病的影响。(C)2011 Wiley Periodicals，Inc. Int J Imaging Syst Technol，21，232-240，2011;在线发表于Wiley Online Library（wileyonlinelibrary.com）。DOI 10.1002/ima.20284
Regional modulation of the level of cortical neurotransmitters in the brain would serve as a new functional brain mapping technique to interrogate the neurochemical actions of the brain. We investigated the utility of the application of low-intensity, pulsed sonication of focused ultrasound (FUS) to the brain to modulate the extracellular level of dopamine (DA) and serotonin (5-HT). FUS was delivered to the thalamic areas of rats, and extracellular DA and 5-HT were sampled from the frontal lobe using the microdialysis technique. The concentration changes of the sampled DA and 5-HT were measured through high-performance liquid chromatography. We observed a significant increase of the extracellular concentrations of DA and 5-HT in the FUS-treated group as compared with those in the unsonicated group. Our results provide the first direct evidence that FUS sonication alters the level of extracellular concentration of these monoamine neurotransmitters and has a potential modulatory effect on their local release, uptake, or degradation. Our findings suggest that the pulsed application of FUS offers new perspectives for a possible noninvasive modulation of neurotransmitters and may have diagnostic as well as therapeutic implications for DA/5-HT-mediated neurological and psychiatric disorders. (C) 2011 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 21, 232-240, 2011; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/ima.20284