A microprocessor controlled mass spectrometer for the fully automated purification and isotopic analysis of breath carbon dioxide.
A microprocessor controlled mass spectrometer for the fully automated purification and isotopic analysis of breath carbon dioxide.
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微处理器控制的质谱仪，用于呼吸二氧化碳的全自动纯化和同位素分析。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Klein
中科院分区：
文献类型：
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作者：
Dale A. Schoeller;P. Klein
衡量13C标记底物氧化利用的呼气测试已被证明在临床上有用，但由于呼气样本的同位素分析缓慢且昂贵，未能得到广泛接受。因此，我们开发了一套全自动、微处理器控制的二氧化碳净化和同位素分析系统。呼气中的二氧化碳通过-94摄氏度和-196摄氏度的冷凝器分别冷凝水和二氧化碳进行低温净化。将二氧化碳引入双进样峰步进质谱仪，并与已知标准进行比较，分析其同位素含量。在没有操作员干预的情况下可以分析30个样品。每个样品平均分析时间为14分钟，分析精度为0.3%，相当于每10(6)份二氧化碳中多出3份13C。分析速度与闪烁计数相当，并允许第二天报告临床呼气测试结果。该方法的精密度足以用于临床应用，因为它在基础呼气二氧化碳中的同位素变化小于0.7%。
Breath tests that measure the oxidative utilization of 13C labeled substrates have been shown to be clinically useful, but have failed to gain wide acceptance because of the slow and costly isotopic analysis of the breath samples. Therefore we have developed a fully automated, microprocessor controlled CO2 purification and isotopic analysis system. The breath CO2 is cryogenically purified by passage through cold traps of -94 degrees C and -196 degrees to condense water and CO2, respectively. The CO2 is intorduced into a dual inlet, peak-stepping mass spectrometer and analyzed for isotopic content by comparison with a known standard. Thirty samples can be analyzed without operator intervention. Analysis time average 14 minutes per sample, and the analysis has a precision of 0.3% which corresponds to 3 parts excess 13C per 10(6) parts CO2. The speed of analysis is comparable with scintillation counting and permits next day reporting of clinical breath test results. The precision is sufficient for clinical applications as it is less than 0.7% isotopic variation in basal breath CO2.