Upregulation of the chemokine monocyte chemoattractant protein-1 following unilateral nerve injury in the peripheral taste system
Upregulation of the chemokine monocyte chemoattractant protein-1 following unilateral nerve injury in the peripheral taste system
复制标题
外周味觉系统单侧神经损伤后趋化因子单核细胞趋化蛋白-1的上调
DOI：
10.1016/j.neulet.2006.11.042
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发表时间：
2007-02-21
影响因子：
2.5
通讯作者：
McCluskey, Lynnette Phillips
中科院分区：
文献类型：
--
作者：
Cavallin, Melissa Ann;McCluskey, Lynnette Phillips
巨噬细胞在单侧鼓室索（CT）神经损伤后被招募到舌头两侧。将这些巨噬细胞招募到外周味觉系统的机制尚不清楚。其他系统的神经退化导致小分子的上调，这些小分子起着趋化因子或趋化因子的作用。趋化因子单核细胞趋化蛋白(MCP)-1和巨噬细胞炎症蛋白(MIP)-1 α是巨噬细胞向感染和损伤部位募集的重要调节因子。我们假设CT神经切片导致双侧MCP-1和MIP-1 α上调。我们用酶联免疫吸附法（ELISA）检测了大鼠单侧CT切片后几个时间点舌部MCP-1和MIP-1 α的蛋白水平。MCP-1在切片后12小时和损伤后24-48小时在舌侧的表达均显著上调。然而，CT神经切片后MIP-1 α表达无明显变化。这些数据表明，趋化因子在神经损伤后受到差异调节，MCP-1可能参与双侧巨噬细胞对神经损伤的反应。此外，MCP-1的增加甚至发生在未受伤的远处部位，并且可能是在切片后对侧CT神经反应缺陷的上游。(c) 2006年由爱思唯尔爱尔兰有限公司出版
Macrophages are recruited to both sides of the tongue following unilateral chorda tympani (CT) nerve injury. The mechanisms responsible for recruiting these macrophages to the peripheral taste system are unknown. Neural degeneration in other systems leads to the upregulation of small molecules that function as chemoattractant cytokines, or chemokines. The chemokines monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-1 alpha are important regulators of macrophage recruitment to sites of infection and injury. We hypothesized that CT nerve sectioning leads to a bilateral upregulation of MCP-1 and MIP-1 alpha. We examined lingual protein levels of MCP-1 and MIP-1 alpha by enzyme-linked immunosorbent assays (ELISA)s at several time points after unilateral CT section in rats. MCP-1 was significantly upregulated on the intact side of the tongue at 12 h after sectioning, and on the injured side at 24-48 h post-injury. However, MIP-1 alpha expression did not significantly change following CT nerve sectioning. These data indicate that chemokines are differentially regulated following neural injury, and that MCP-1 may contribute to the bilateral macrophage response to neural injury. Furthermore, the increase in MCP-1 occurs even in uninjured, distant sites, and may be upstream from the deficits in neural responses from the contralateral CT after sectioning. (c) 2006 Published by Elsevier Ireland Ltd.