Glucocorticoid receptor homeostasis and renal glutaminase activity.
Glucocorticoid receptor homeostasis and renal glutaminase activity.
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糖皮质激素受体稳态和肾谷氨酰胺酶活性。
DOI：
10.1159/000423389
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发表时间：
1994
影响因子：
--
通讯作者：
Welbourne,TC
中科院分区：
文献类型：
--
作者：
Nam,M;Welbourne,TC
材料和方法我们的策略是在体内通过配体的可用性和GR基因的自调节来控制GR种群，在体外通过将GR基因导入近端小管样培养的肾细胞来增加内源性的GR种群。体内实验以体重300-350 g的雄性Sprague-Dawley大鼠为实验对象。肾上腺切除术（ADX）通过背侧入路（Harlan, Indianapolis, Ind， USA）进行，动物在研究前维持0.9% NaCl。试验均在ADX后12 d内进行。Gc以皮下微丸的形式给药，以恒定的日剂量（Innovative Products, Toledo, Ohio， USA）释放曲安奈德，分别为0.40或40µg/ 100g bw，低剂量或高剂量。
Materials and MethodsOur strategy was to manipulate the GR population in vivo through ligand availability and hence autoregulation of the GR gene and in vitro by bolstering the endogenous population by introducing the GR gene into proximal tubule-like cultured kidney cells. In vivo experiments were performed on male Sprague-Dawley rats weighing 300-350 g. Adrenalectomies (ADX) were performed via the dorsal approach (Harlan, Indianapolis, Ind., USA) with the animals maintained on 0.9% NaCl prior to study. Experi-ments were always carried out within 12 days of ADX. Gc was administered in the form of a subdermal pellet releasing triamcinolone at a constant daily rate (Innovative Products, Toledo, Ohio, USA) of either 0.40 or 40 µg/100 g bw, low or high dose, respectively.