Effective Antibacterial Activity of Degradable Copper-Doped Phosphate-Based Glass Nanozymes
Effective Antibacterial Activity of Degradable Copper-Doped Phosphate-Based Glass Nanozymes
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DOI：
10.1021/acsami.0c22746
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发表时间：
2021-03-09
影响因子：
9.5
通讯作者：
Liu, Jianfeng
中科院分区：
文献类型：
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作者：
Liu, Yifan;Nie, Ning;Liu, Jianfeng
含铜抗菌剂由于其众所周知的高抗菌功效而在医用消毒剂领域中具有很高的价值。通过人工合成纳米酶来提高细菌系统中活性氧的水平已成为研究热点。在此，我们描述了可降解的铜掺杂磷酸盐基玻璃（Cu-PBG）纳米酶的设计和制备，它可以实现对革兰氏阳性和革兰氏阴性细菌的优良的抗菌效果。其抗菌机理是通过产生活性氧风暴和释放铜离子来实现的。它在酸性伤口中表现得像过氧化物酶，并通过催化H2 O2分解为羟基自由基（（OH）-O-中心点）对细菌施加致命的氧化应激。与任何其他报道的纳米酶完全不同，Cu-PBG由于其磷酸盐玻璃性质而本质上可降解。它在生理环境中逐渐降解并释放铜离子，这进一步提高了抑制效率。在体外和体内均证实了满意的抗菌效果。由于可生物降解，所制备的Cu-PBG具有优异的体内生物相容性，并且不会因其在活生物体中的长时间停留时间而引起任何不良影响。总的来说，这些结果表明，Cu-PBG纳米酶可用作具有巨大临床转化潜力的有效含铜抗菌剂。
Copper-containing antimicrobials are highly valuable in the field of medical disinfectants owing to their well-known high antimicrobial efficacy. Artificially synthesized nanozymes which can increase the level of reactive oxygen species (ROS) in the bacterial system have become research hotspots. Herein, we describe the design and fabrication of degradable Cu-doped phosphate-based glass (Cu-PBG) nanozyme, which can achieve excellent antibacterial effects against Gram-positive and Gram-negative bacteria. The antibacterial mechanism is based on the generation of ROS storm and the release of copper. It behaves like a peroxidase in wounds which are acidic and exerts lethal oxidative stress on bacteria via catalyzing the decomposition of H2O2 into hydroxyl radicals ((OH)-O-center dot). Quite different from any other reported nanozymes, the Cu-PBG is intrinsically degradable due to its phosphate glass nature. It gradually degrades and releases copper ions in a physiological environment, which further enhances the inhibition efficiency. Satisfactory antibacterial effects are verified both in vitro and in vivo. Being biodegradable, the prepared Cu-PBG exhibits excellent in vivo biocompatibility and does not cause any adverse effects caused by its long-time residence time in living organisms. Collectively, these results indicate that the Cu-PBG nanozyme could be used as an efficient copper-containing antimicrobial with great potential for clinical translation.