Expression of RUNX3 protein in human lung adenocarcinoma: Implications for tumor progression and prognosis
Expression of RUNX3 protein in human lung adenocarcinoma: Implications for tumor progression and prognosis
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DOI：
10.1111/j.1349-7006.2005.00033.x
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发表时间：
2005-04-01
期刊：
影响因子：
5.7
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Araki, K;Osaki, M;Ito, H
Runt相关转录因子3属于转录因子的Runt结构域家族，在正常组织发育和几个器官的肿瘤发生中起关键作用。我们将我们的注意力集中在人肺AC和非肿瘤性肺组织中RUNX3蛋白的表达，并将结果与临床病理特征进行比较。我们评估了RUNIX 3蛋白在17对肺AC和非肿瘤肺组织中的表达。此外，研究了98个肺AC以检查RUNX3阳性细胞的频率。Western印迹分析显示，在所有17个AC和非肿瘤组织中，在45 kDa处有一条带。免疫组化显示肺泡II型肺细胞或克拉拉细胞的免疫反应。RUNX3在有BAC成分的癌组织中的表达明显高于无BAC成分的癌组织（P < 0.01）。较低的RUNX3水平与低分化类型相关（P=0.049）。50例RUNIX 3表达水平较高的患者的5年生存率显著高于48例RUNIX 3表达水平较低的患者（P = 0.027）。RUNX3蛋白在肺AC中的表达可能在肿瘤的进展和患者的生存中起关键作用。
Runt-related transcription factor 3 belongs to the runt domain family of transcription factors that play a pivotal role during normal tissue development and tumorigenesis in several organs. We directed our attention to the expression of RUNX3 protein in human lung AC and non-neoplastic lung tissues, comparing the results with clinicopathological profiles. We evaluated the expression of RUNIX3 protein in 17 pairs of lung AC and non-neoplastic lung tissue. Furthermore, 98 lung AC were studied to examine the frequency of RUNX3-positive cells. Western blot analysis showed a single band at 45 kDa in all 17 AC and non-neoplastic tissues. Immunohistochernistry revealed immunoreactivity in alveolar type II pneumocytes or Clara cells. RUNX3 was expressed more frequently in the carcinomas with a BAC component than in those without (P < 0.01). Lower RUNX3 levels were associated with poorly differentiated types (P=0.049). The five-year survival rate was significantly higher in the 50 patients with higher levels of RUNIX3 expression than in the 48 patients with lower levels (P = 0.027). The expression of RUNX3 protein in lung AC might play a pivotal role in tumor progression and patients' survival.