Caveolin-1 expression in advanced-stage ovarian carcinoma - A clinicopathologic study
Caveolin-1 expression in advanced-stage ovarian carcinoma - A clinicopathologic study
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DOI：
10.1006/gyno.2001.6156
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发表时间：
2001-05-01
影响因子：
4.7
通讯作者：
Reich, R
中科院分区：
文献类型：
--
作者：
Davidson, B;Nesland, JM;Reich, R
目标。本研究的目的是分析晚期卵巢癌组织中小凹蛋白-1的表达及小窝蛋白成分与预后的关系。应用免疫组织化学方法检测了45例晚期卵巢癌(FIGO分期III~IV期)患者的76例原发卵巢癌和45例转移灶组织中小窝蛋白-1的表达。根据疾病结果将患者分为长期幸存者和短期幸存者。另外对20例非肿瘤性输卵管和卵巢进行了研究。平均随访期为70个月。长期生存者的无病生存期和总生存期的平均值分别为109个月和125个月，而短期生存者分别为3个月和21个月。Caveolin-1表达定位于细胞膜24/76(32%)，胞浆52/76(68%)。与原发肿瘤相比，这两种模式在转移性肿瘤中更常见。此外，在短期存活者的肿瘤中，膜免疫反应更常见。这些差异无统计学意义(P>；0.05)，17/20(85%)的非肿瘤性病变可见胞膜和胞浆联合免疫反应。尽管在体外研究中，小凹蛋白-1在酪氨酸激酶介导的信号转导中发挥作用，但癌组织中小窝蛋白-1的表达与c-erbB-2和表皮生长因子受体的蛋白表达没有相关性，这是先前对该患者进行的一项研究的评估。这项研究首次提供了小窝蛋白-1膜在人类恶性肿瘤中表达的体内证据。小窝蛋白-1在晚期卵巢癌中经常表达，但似乎不是这些肿瘤的疾病预后的有力预测指标。与非肿瘤上皮相比，癌组织中表达水平的降低可能表明小窝蛋白-1作为肿瘤抑制基因的作用，(C)2001年学术出版社。
Objective. The aim of this study was to analyze the correlation among the expression of caveolin-1, the protein constituent of caveolae, and disease outcome in advanced-stage ovarian carcinomas.Methods. Sections from 76 primary ovarian carcinomas and metastatic lesions from 45 patients diagnosed with advanced-stage ovarian carcinoma (FIGO stages III-IV) were evaluated for caveolin-1 expression using immunohistochemistry. Patients were divided into long-term survivors and short-term survivors based on disease outcome. Twenty nonneoplastic fallopian tubes and ovaries were additionally studied.Results. The mean follow-up period was 70 months. The mean values for disease-free survival and overall survival were 109 and 125 months for long-term survivors, compared to 3 and 21 months for short-term survivors, respectively. Caveolin-1 expression was localized to the cell membrane in 24/76 (32%) specimens and was detected in the cytoplasm in 52/76 (68%) cases. Both patterns were more often detected in metastases, when compared with primary tumors. In addition, membrane immunoreactivity was more often seen in tumor of short-term survivors. These differences did not reach statistical significance (P > 0.05), Combined membrane and cytoplasmic immunoreactivity was seen in 17/20 (85%) nonneoplastic lesions. Despite its role in tyrosine-kinase-mediated signal transduction in vitro studies, caveolin-1 expression in carcinomas showed no association with the protein expression of c-erbB-2 and epidermal growth factor receptor, evaluated in a previous study of this patient cohort.Conclusions. This study provides the first in vivo evidence of caveolin-1 membrane expression in human malignancies. Caveolin-1 is often expressed in advanced-stage ovarian carcinoma, but does not appear to be a powerful predictor of disease outcome in these tumors. The reduced expression level in carcinomas compared to nonneoplastic epithelium may point to a role for caveolin-1 as a tumor suppressor gene, (C) 2001 Academic Press.