TRANSFORMATIONAL PROCESS OF THE ENDOSOMAL COMPARTMENT IN NEPHROCYTES OF DROSOPHILA-MELANOGASTER
TRANSFORMATIONAL PROCESS OF THE ENDOSOMAL COMPARTMENT IN NEPHROCYTES OF DROSOPHILA-MELANOGASTER
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DOI：
10.1007/bf00309878
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发表时间：
1990-11-01
影响因子：
3.6
通讯作者：
IKEDA, K
中科院分区：
文献类型：
--
作者：
KOENIG, JH;IKEDA, K
本研究通过电子显微镜研究果蝇肾细胞（加兰细胞）内体区室的转化过程，该过程发生在内化物质的内吞加工过程中。加兰细胞的内体区室由皮质细胞质中突出的管状/空泡复合物组成。当在29 ℃下封闭被包覆的凹坑的内化时，C使用内吞作用突变体shibirets，在29 ℃ 7分钟后小管逐渐消失。C. 在29 ℃下加热12分钟。C，空泡也消失了。因此，内体区室似乎不断经历一个转化过程，需要不断补充包被囊泡。这些数据表明，内体隔室的管状组分通过管状膜的扩张逐渐转化为空泡。然后液泡通过内陷而发生转变，形成扁平的囊泡。液泡腔内的电子透明物质似乎通过内陷膜被挤出到细胞质中。未发现穿梭载体（如囊泡或小管）可能参与将内吞物质和膜从内体隔室转运至溶酶体或返回质膜。
This study investigates by electron miroscopy the transformational process of the endosomal compartment of the Drosophila nephrocyte, the garland cell, which occurs during endocytotic processing of internalized material. The endosomal compartment of the garland cell consists of a prominent tubular/vacuolar complex in the cortical cytoplasm. When internalization of coated pits is blocked at 29.degree. C using the endocytosis mutant, shibirets, the tubules gradually disappear after 7 min at 29.degree. C. By 12 min at 29.degree. C, the vacuoles also disappear. Thus, the endosomal compartment appears to constantly undergo a transformational process that necessitates continuous replenishment by coated vesicles. The data suggest that the tubular component of the endosomal compartment gradually transforms into vacuoles by the expansion of the tubular membrane. The vacuoles then transform by invaginating into themselves, creating flattened cisternae. The electron-lucent substance in the lumina of the vacuoles appears to be extruded into the cytoplasm through the invaginating membrane. No shuttle vehicles such as vesicles or tubules could be identified that might have been involved in the transporting of endocytosed materials and membrane from the endosomal compartment to lysosomes or back to the plasma membrane.