Photoactive Poly(ethylene glycol) Organosilane Films for Site-Specific Protein Immobilization
Photoactive Poly(ethylene glycol) Organosilane Films for Site-Specific Protein Immobilization
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用于位点特异性蛋白质固定的光活性聚乙二醇有机硅烷薄膜
作者：
Delana A. Nivens;D. Conrad
我们描述了一种新型光活性有机硅烷的合成、表征和使用，该有机硅烷用于蛋白质的光固定化。采用原子力显微镜、椭偏光谱和衰减全反射-傅里叶变换红外光谱对2-硝基-5-[11-（三甲氧基硅基）十一烷基]氧苄基甲氧基聚乙二醇丙酸酯薄膜进行了表征。当这种有机硅烷用于修饰硅衬底时，沉积过程中使用的溶剂会影响所得薄膜的表面浓度和形貌。尽管用含水缓冲液或无水甲苯沉积的膜都可以用来将蛋白质附着在表面上，但用含水缓冲液沉积的膜显示出的非特异性蛋白质吸附比用甲苯沉积的膜少4 - 6倍。用这种类型的光活性和蛋白质“拒绝”膜修饰表面应该有助于高分辨率蛋白质微阵列的发展。
We describe the synthesis, characterization, and use of a new photoactive organosilane developed for the photoimmobilization of proteins. Thin films of 2-nitro-5-[11-(trimethoxysilyl)undecyl]oxybenzyl methoxy poly(ethylene glycol) propanoate are characterized using atomic force microscopy, spectroscopic ellipsometry, and attenuated total reflection-Fourier transform infrared spectroscopy. When this organosilane is used to modify silicon substrates, the solvent used during deposition affects the surface concentration and morphology of the resultant film. Although films deposited from either aqueous buffer or anhydrous toluene can be used to attach proteins to surfaces, films deposited from aqueous buffer display 4−6 times less nonspecific protein adsorption that those deposited from toluene. Modification of surfaces with this type of photoactive and protein “rejecting” film should aid in the development of high-resolution protein microarrays.