Effects of three intravenous lipid emulsions on the survival and mononuclear phagocyte function of septic rats
Effects of three intravenous lipid emulsions on the survival and mononuclear phagocyte function of septic rats
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DOI：
10.1016/s0899-9007(02)00830-4
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发表时间：
2002-09-01
期刊：
影响因子：
4.4
通讯作者：
Jiménez-Jiménez, FJ
中科院分区：
文献类型：
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作者：
Garnacho-Montero, J;Ortiz-Leyba, C;Jiménez-Jiménez, FJ
目的：静脉注射脂肪乳剂的免疫抑制作用是一个备受关注和争论的问题。在革兰氏阴性菌血症的大鼠模型中，我们评估了三种不同三酰甘油成分的静脉注射脂肪乳剂的使用是否会影响死亡率、细菌清除量和前列腺素E-2(PGE(2))水平，并将这些组与口服大鼠和以葡萄糖形式摄入少量热量的大鼠进行比较(饥饿)。方法：大鼠被分配到五组之一：第一组(对照组，n=15)接受啮齿动物食物和生理盐水；第二组(饥饿组，n 12)不能获得食物和接受。实验分为4组，每组12例，分别给予5%葡萄糖、17例含长链三酰甘油的全胃肠外营养(TPN)、12例含中、长链三酰甘油的TPN、15例含橄榄油乳剂的TPN。动物接受等氮。和等热量的全胃肠外营养。TPN 2d后，经静脉导管注入108个集落形成单位的大肠杆菌；2d后处死动物。取血、脾、肝、肺进行培养。结果：实验3组和实验4组大鼠肝脏和肺组织细菌生长均显著高于实验组。1，其他组间无差异。3、4组菌血症发生率明显高于1组，其他组间差异无统计学意义。血浆PGE(2)水平无差异，死亡率未受影响。结论：与使用长链三酰甘油或中加长链三酰甘油的TPN大鼠相比，口服喂养的对照组大鼠的细菌清除明显保持。然而，使用静脉注射富含油酸的脂肪乳剂是减少这种紊乱的有效方法，尽管血浆PGE(2)水平和存活率没有改变。(C)爱思唯尔科学公司，2002年。
OBJECTIVES: The immunosuppressive effects of intravenous lipid emulsions are a matter of great concern and debate. In a rat model of gram-negative bacteremia, we assessed whether the use if three intravenous lipid emulsions with different triacylglycerol compositions, could influence mortality, bacterial clearance, and prostaglandin E-2 (PGE(2)) levels and compared these groups with groups of orally-fed rats and rats that, received a small amount of calories in form of glucose without enteral feeding (starvation).METHODS: Rats were assigned to one of five groups: group 1 (control, n = 15) received rodent chow ad libitum and saline infusion; group 2 (starvation group, n 12) had no access to chow and,received. infusion of 5% glucose; group 3 (n = 17) received total parenteral nutrition (TPN) with long-chain triacylglycerols; group 4 (n = 12) received TPN with medium- and long-chain triacylglycerols; and group 5 (n = 15) received TPN with its emulsion based on olive oil. Animals received isonitrogenous. and isocaloric TPN. After 2 d of TPN, a dose of 108 colony-forming units of Escherichia coli was introduced, via the venous catheter; 2 d later the animals were killed. Blood, spleen, liver, and lungs were cultured. Circulating levels of PGE(2) were measured.RESULTS: Bacterial growth in the liver and lunge were significantly higher in groups 3 and 4 than,in group. 1, with no differences among the other groups. Rates of bacteremia were significantly higher in groups 3 and 4 than in group 1, with no differences among-the other groups. Plasma levels of PGE(2) did not differ, and mortality was unaffected.CONCLUSIONS: Bacterial clearance clearly was preserved in orally fed, control rats when compared with rats on TPN with long-chain triacylglycerols or medium- plus long-chain, triacylglycerols. However, the use of a lipid emulsion enriched intravenously with oleic acid Was a valid Way of reducing this disturbance, although plasma levels of PGE(2) and survival were not modified. (C) Elsevier Science Inc. 2002.