Lithium inhibits apoptosis of mouse neural progenitor cells
Lithium inhibits apoptosis of mouse neural progenitor cells
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DOI：
10.1097/00001756-200310060-00004
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发表时间：
2003-10-06
期刊：
影响因子：
1.7
通讯作者：
Senda, T
中科院分区：
文献类型：
--
作者：
Shimomura, A;Nomura, R;Senda, T
作为中枢神经系统中未分化的前体细胞，神经祖细胞（NPC）提供新的神经元和神经胶质细胞来修复成人脑内的损伤。最近，发现NPC经历凋亡。在无血清碱性成纤维细胞生长因子培养基中，原代培养的神经上皮细胞表达巢蛋白，因此可能被认为是NPC。电镜观察和TUNEL法检测显示这些NPC发生凋亡。用锂（糖原合成酶激酶3 β（GSK 3 β）的特异性抑制剂）处理NPC可显著抑制细胞凋亡。在锂未处理或处理的NPC中未检测到促凋亡蛋白酶caspase-3的活性。这些发现表明，锂可以通过抑制caspase-3非依赖性凋亡途径来保护NPC免受凋亡。
As undifferentiated precursor cells in the CNS, neural progenitor cells (NPCs) supply new neurons and glial cells to repair damage within the adult brain. Recently, NPCs were found to undergo apoptosis. In serum-free basic fibroblast growth factor-containing culture medium, primary culture cells from fetal mouse neuroepithelium expressed nestin, and thus might be regarded as NPCs. These NPCs demonstrated apoptosis under electron microscopy and TUNEL assay. Treatment of NPCs with lithium, a specific inhibitor of glycogen synthase kinase 3beta (GSK3beta), significantly suppressed apoptosis. Activity of pro-apoptotic protease caspase-3 was not detected in either lithium-untreated or-treated NPCs. These findings suggest that lithium may protect NPCs against apoptosis by inhibiting caspase-3-independent apoptotic pathways.